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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (19): 56-63.doi: 10.11924/j.issn.1000-6850.casb2024-0533

• 林学·园艺·园林 • 上一篇    下一篇

鹿药种子的无菌萌发及组织培养研究

韩红亮1,2(), 程蒙1, 李颖1, 陈宝妮3, 叶嘉敏3, 李洁4, 纪瑞锋1, 周修腾1()   

  1. 1 中国中医科学院中药资源中心/道地药材品质保障与资源持续利用全国重点实验室，北京 100700
    2 陕西国际商贸学院，西安 710000
    3 广东药科大学，广州 510000
    4 云南山水环保工程有限公司，昆明 650000
  • 收稿日期:2024-08-27 修回日期:2024-10-15 出版日期:2025-07-05 发布日期:2025-07-10
  • 通讯作者:
    周修腾，男，1986年出生，广东潮汕人，副研究员，博士，主要从事药用植物寄生与共生等研究工作。通信地址：100700 北京市东城区东直门内南小街16号 中国中医科学院中药资源中心，Tel：010-13522661277，E-mail：。
  • 作者简介:

    韩红亮，女，1996年出生，甘肃天水人，硕士研究生，研究方向：中药质量标准的研究。通信地址：710000 陕西省西安市西咸新区沣西新城大学园统一西路35号，Tel：029-15625042521，E-mail：。

  • 基金资助:
    中国中医科学院科技创新工程项目“‘一带一路’药用植物种质资源收集目录研究”(CI2021B014)

Maianthemum japonicum Seeds: Aseptic Germination and Tissue Culture

HAN Hongliang1,2(), CHENG Meng1, LI Ying1, CHEN Baoni3, YE Jiamin3, LI Jie4, JI Ruifeng1, ZHOU Xiuteng1()   

  1. 1 State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Material Medical, China Academy of Chinese Medical Sciences, Beijing 100700
    2 Shaanxi Institute of International Business, Xi’an 710000
    3 Guangdong Pharmaceutical University, Guangzhou 510000
    4 Yunnan Shanshui Environmental Protection Engineering Co., Ltd., Kunming 650000
  • Received:2024-08-27 Revised:2024-10-15 Published:2025-07-05 Online:2025-07-10

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摘要： 为建立鹿药种子无菌萌发及组织培养的方法，以鹿药种子为试验材料，采用正交试验探究外源激素种类、浓度及浸泡时间对种子萌发的影响，同时用鹿药茎段进行愈伤组织培养获得无菌苗，最后在温室引种栽培鹿药。结果表明，100 mg/L的GA3、500 mg/L的乙烯利对鹿药种子的萌发过程有促进作用，并且浸泡48 h的效果优于浸泡12、24 h。鹿药茎段愈伤组织处理中，次氯酸钠溶液消毒和切除黑色物质顺序交换后，鹿药污染率降低，褐变率没有显著差异。在适宜温度下，鹿药能迅速打破休眠进行生长。本研究发现不同外源激素处理的鹿药种子发芽率并无显著差异，但外源激素对其萌发具有一定促进作用，且鹿药茎段愈伤组织诱导褐变率较高。

关键词: 鹿药, 种子, 无菌萌发, 组织培养, 引种栽培

Abstract:

In order to establish the method of aseptic germination and tissue culture of Maianthemum japonicum seeds, the seeds of M. japonicum were used as experimental materials, and the effects of exogenous hormone types, concentrations and soaking time on seed germination were investigated by orthogonal test. At the same time, the stems of Cervus elaphus were used for callus culture to obtain sterile seedlings. Finally, M. japonicum was introduced and cultivated in greenhouse. The results showed that 100 mg/L GA3 and 500 mg/L ethephon could promote the growth process of M. japonicum seeds, and the effect of soaking for 48 h was better than that of soaking for 12 h and 24 h. In the treatment of callus of stem section of M. japonicum, after the sequential exchange of sodium hypochlorite solution disinfection and black material removal, the pollution rate of M. japonicum decreased, and there was no significant difference in browning rate. At a suitable temperature, M. japonicum can quickly break dormancy and grow. This study preliminarily clarified that there was no significant difference in the germination rate of M. japonicum seeds treated with different exogenous hormones, but it had a certain promoting effect on their germination, and the callus induction browning rate of M. japonicum stem segments was higher, which could provide reference for the rapid propagation of M. japonicum in the later stage.

Key words: Maianthemum japonicum, seed, sterile germination, tissue culture, introduction and cultivation