穿心莲NAC基因家族的鉴定与表达分析

doi:10.11924/j.issn.1000-6850.casb2024-0755

中国农学通报 ›› 2024, Vol. 40 ›› Issue (36): 117-125.doi: 10.11924/j.issn.1000-6850.casb2024-0755

穿心莲NAC基因家族的鉴定与表达分析

徐世强¹^,²(), 李静宇¹^,², 孙铭阳¹^,², 顾艳¹^,², 王继华¹^,²()

¹ 广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室，广州 510640
² 广东省道地南药资源保护与利用工程中心，广州 510640

收稿日期:2023-11-08 修回日期:2024-05-15 出版日期:2024-12-25 发布日期:2024-12-23
通讯作者:
王继华，1979年出生，湖北荆门人，研究员，博士，研究方向：南药资源保护与利用。通信地址：510641广东省广州市天河区五山路金颖西二街18号广东农科院作物所，Tel：020-87541262，E-mail：wangjihua@gdaas.com。
作者简介:
徐世强，男，1989年出生，助理研究员，博士，研究方向：中草药功能基因组学。通信地址：510641 广东省广州市天河区五山路金颖西二街18号广东农科院作物所，Tel：020-87541262，E-mail：shiqxu0376@163.com。
基金资助:
广东省农业科学院创新基金“穿心莲ApNAC72-like转录因子调控干旱和高温胁迫的分子机制”(2023); 广东省重点领域研发计划项目“广藿香等6种岭南中药材新品种培育研究”(2020B020221001)

Identification and Expression Analysis of NAC Gene Family in Andrographis paniculate

XU Shiqiang¹^,²(), LI Jingyu¹^,², SUN Mingyang¹^,², GU Yan¹^,², WANG Jihua¹^,²()

¹ Crop Research Institute, Guangdong Academy of Agriculture Sciences/ Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangzhou 510640
² Guangdong Provincial Engineering & Technology Research Center for Conservation and Utilization of the Genuine Southern Medicinal Resources, Guangzhou 510640

Received:2023-11-08 Revised:2024-05-15 Published:2024-12-25 Online:2024-12-23

摘要/Abstract

摘要：

NAC（包括NAM、ATAF和CUC亚家族）转录因子在植物的生长、发育以及对各种非生物和生物胁迫的响应中发挥重要作用。本研究旨在鉴定和分析穿心莲中的NAC基因家族，通过生物信息学方法鉴定了穿心莲中的NAC基因家族，并对其理化性质、进化关系、顺式作用元件、染色体位置分布、共线性以及表达谱进行了详细分析。共鉴定出91个穿心莲NAC基因，蛋白长度为139~715 aa，不均匀分布在染色体两端；亚细胞定位预测发现绝大多数基因定位在细胞核中；通过和拟南芥NAC蛋白构建进化树，将其分为16个亚家族；物种内共线性分析发现穿心莲NAC中有4组串联复制和36对片段复制基因对；基于亚家族功能保守性，顺式作用元件分析以及干旱胁迫和MeJA处理转录组分析，挖掘了响应穿心莲干旱和穿心莲内酯合成的NAC基因。本研究对穿心莲的NAC基因家族进行了鉴定和分析，预测了干旱和内酯合成相关基因，为后续NAC基因功能研究奠定了基础。

关键词: 穿心莲, NAC基因家族, 干旱胁迫, MeJA处理, 全基因组鉴定, 生物信息学, 理化性质, 进化树, 顺式作用元件, 染色体位置分布, 共线性分析, 表达谱分析

Abstract:

NAC (NAM, ATAF, and CUC) transcription factors play a crucial role in plant growth and development, as well as in responses to various abiotic and biotic stresses. This study aims to identify and analyze the NAC gene family in Andrographis paniculata. Using bioinformatics methods, NAC genes were identified from the A.paniculata genome, followed by analysis of their phylogenetic relationships, cis-acting elements, chromosome distribution, collinearity, and expression profiles under drought stress and MeJA treatment. A total of 91 NAC genes were identified in A.paniculata, with protein lengths ranging from 139 to 715 amino acids, unevenly distributed at both ends of the chromosomes. Subcellular localization predictions revealed that most of the genes were located in the nucleus. Phylogenetic analysis, based on the construction of a phylogenetic tree with Arabidopsis NAC proteins, divided these genes into 16 subfamilies. Intraspecies collinearity analysis identified 4 tandem duplication groups and 36 segmental duplication gene pairs within the A.paniculata NAC family. Based on the functional conservation of subfamilies, analysis of cis-acting elements, and transcriptome analysis under drought stress and MeJA treatment, NAC genes responsive to drought and andrographolide synthesis were identified. This study identified and analyzed the NAC gene family in A.paniculata, predicting genes related to drought response and andrographolide synthesis, laying the foundation for further functional research on NAC genes.

Key words: Andrographis paniculata, NAC gene family, drought stress, MeJA treatment, whole-genome identification, bioinformatics, physicochemical properties, phylogenetic tree, cis-acting elements, chromosome distribution, collinearity analysis, expression profiles analysis

徐世强, 李静宇, 孙铭阳, 顾艳, 王继华. 穿心莲NAC基因家族的鉴定与表达分析[J]. 中国农学通报, 2024, 40(36): 117-125.

XU Shiqiang, LI Jingyu, SUN Mingyang, GU Yan, WANG Jihua. Identification and Expression Analysis of NAC Gene Family in Andrographis paniculate[J]. Chinese Agricultural Science Bulletin, 2024, 40(36): 117-125.

图/表 6

图1. 穿心莲和拟南芥NAC基因进化树内圈绿色五角星和红色圆形分别表示穿心莲和拟南芥NAC基因，不同颜色外圈对应标注各亚家族名称

图2. 穿心莲NAC基因启动子区域顺式作用元件

图3. 穿心莲NAC染色体位置分布

图4. 穿心莲NAC共线性分析（a）物种内共线性分析红色线段表示NAC片段复制基因对；（b）物种间共线性分析；红色线段表示两物种间NAC共线性对

图5. 穿心莲NAC基因响应干旱胁迫和MeJA处理转录组表达分析

图6. 穿心莲NAC基因qPCR表达谱

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穿心莲NAC基因家族的鉴定与表达分析

Identification and Expression Analysis of NAC Gene Family in Andrographis paniculate

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