欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (19): 82-93.doi: 10.11924/j.issn.1000-6850.casb2025-0905

• 林学·园艺·园林 • 上一篇    下一篇

激素代谢组及转录组联合分析蓝光对三叶青块根膨大发育的调控机制

赵刚1(), 王悦2, 朱海1, 段华平1, 张莉1, 刘萍3, 闻静1(), 郭连金1()   

  1. 1 上饶师范学院， 江西上饶 334001
    2 黑河海关综合技术中心， 黑龙江黑河 164000
    3 江西三叶农业科技发展有限公司， 江西玉山 334700
  • 收稿日期:2025-11-05 修回日期:2026-06-16 出版日期:2026-10-15 发布日期:2026-10-15
  • 通讯作者:
    闻静，女，1983年出生，黑龙江哈尔滨人，教授，博士，主要从事药用植物高山有机栽培及药用植物次生代谢工程研究。通信地址：334001 江西省上饶市信州区志敏大道401号，Tel：0793-8153721，E-mail：。
    郭连金，男，1976年出生，山东滨州人，教授，硕士，主要从事药用植物种质资源及药用植物次生代谢工程。通信地址：334001 江西省上饶市信州区志敏大道401号，Tel：0793-8153721，E-mail：。
  • 作者简介:

    赵刚，男，1982年出生，黑龙江哈尔滨人，实验师，学士，主要从事药用植物次生代谢工程及生物大数据分析。通信地址：334001 江西省上饶市信州区志敏大道401号，Tel：0793-8153721，E-mail：。

  • 基金资助:
    上饶市重点研发计划项目“怀玉山三叶青高山高光效设施栽培及配套生产技术研究与示范”(2021C003); 2025年江西省中医药标准化研究项目“怀玉山三叶青高山设施种苗高效繁殖及块根定向诱导标准化关键技术研究”(2025B03); 江西省教育厅科学技术项目“基于靶向黄酮代谢组分析的LED蓝光对三叶青黄酮类化合物增益效应的研究”(GJJ2201807); 江西省自然科学基金项目“江西抚州中新世植物多样性研究”(20224BAB215003); 上饶市2025基础研究计划“马家柚柚皮主要功效成分绿色提取机制研究”(2025D013)

Integrated Analysis of Hormone Metabolome and Transcriptome to Reveal Regulatory Mechanism of Blue Light on Tuberous Root Expansion in Tetrastigma hemsleyanum

ZHAO Gang1(), WANG Yue2, ZHU Hai1, DUAN Huaping1, ZHANG Li1, LIU Ping3, WEN Jing1(), GUO Lianjin1()   

  1. 1 Shangrao Normal University, Shangrao, Jiangxi 334001
    2 Heihe Customs Comprehensive Technical Center, Heihe, Heilongjiang 164000
    3 Jiangxi Sanye Agricultural Technology Development Co., Ltd., Yushan, Jiangxi 334700
  • Received:2025-11-05 Revised:2026-06-16 Published:2026-10-15 Online:2026-10-15

RichHTML

0

PDF (PC)

3

摘要：

为深入解析蓝光通过调控内源激素水平及其信号通路，促进三叶青块根膨大发育的机制，通过迈维LC-MS/MS平台，定量检测蓝光处理前后三叶青块根中8类植物激素[生长素(IAA)、细胞分裂素(CK)、脱落酸(ABA)、茉莉酸(JA)、水杨酸(SA)、赤霉素(GA)、乙烯(ETH)、独脚金内酯(SL)]及其相关中间体的含量变化，结合转录组测序技术分析差异表达基因，关联激素水平变化与其关键信号通路基因的差异表达，并通过qPCR技术验证目标基因表达量差异，从转录调控与激素代谢协同作用的角度，揭示蓝光对三叶青块根发育的调控机制。激素代谢组学分析显示，IAA水平无显著变化，但其信号通路相关基因表达水平显著下调，表明蓝光条件下IAA对块根膨大的增益作用不显著；蓝光处理后，玉米素糖苷的显著富集可能通过影响蔗糖代谢及淀粉储存，间接促进块根发育；而赤霉素中GA20含量下降及GID基因稳定表达共同导致其信号通路弱化，可能对块根膨大产生正向调控作用。此外，蓝光显著上调茉莉酸含量，同时抑制其信号转导的负调控ThJAZL2663基因的表达，推测蓝光通过增强JA的生理活性，直接促进块根发育。研究认为，低密度蓝光辐射可提高JA含量并抑制ThJAZL2663基因的表达，从而放大JA生理活性，并直接促进三叶青块根发育；蓝光通过差异调控激素网络协同增效，包括弱化赤霉素(GA20-GID)信号通路、诱导细胞分裂素糖苷化代谢等间接调控机制，共同促进了三叶青块根的膨大。本研究不仅解析了蓝光调控内源激素代谢变化对块根发育的影响，更为三叶青蓝光调控补光栽培技术的优化提供了关键理论支撑。

关键词: 激素代谢组, 转录组, 低密度蓝光, 三叶青, 块根发育, 关联比对分析

Abstract:

This study aims to deeply analyze that blue light promotes the expansion and development of tuberous roots of Tetrastigma hemsleyanum by regulating endogenous hormone levels and their signaling pathways. Using the MetWare LC-MS/MS platform, we quantified the changes in the contents of eight major plant hormones, including auxin (IAA), cytokinin (CK), abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), gibberellin (GA), ethylene (ETH), and strigolactone (SL), along with their intermediates in T. hemsleyanum tubers before and after blue light treatment. Combined with transcriptome sequencing to analyze differentially expressed genes, we correlated hormonal changes with the differential expression of key genes in their signaling pathways and validated target genes using qPCR, thereby investigating the effects of blue light on tuber development from the perspective of transcriptional regulation and hormonal metabolic synergy. Hormone metabolomics revealed that while IAA levels showed no significant change, the expression of its signaling-related genes was markedly downregulated, indicating that IAA contributes minimally to tuber expansion under blue light. The significant accumulation of zeatin-O-glucoside following blue light treatment may indirectly promote tuber development through sucrose metabolism and starch storage. Furthermore, the decrease in GA20 content and stable expression of GID genes led to the attenuation of gibberellin signaling, potentially exerting a positive regulatory effect on tuber swelling. Additionally, blue light significantly upregulated JA levels while suppressing the expression of ThJAZL2663, a negative regulator of JA signal transduction, suggesting that blue light directly enhances JA physiological activity to promote tuber development. Low-intensity blue light irradiation increases JA content and inhibits the expression of ThJAZL2663 gene, thereby amplifying JA physiological activity and directly promoting development. Moreover, blue light exerts a synergistic effect through differential regulation of the hormonal network, including weakening the gibberellin (GA20-GID) signaling pathway and inducing cytokinin glycosylation metabolism, to facilitate tuber expansion in T. hemsleyanum. This study not only elucidates the impact of blue light-mediated hormonal metabolic changes on tuber development but also provides critical theoretical support for optimizing blue light-regulated supplemental cultivation techniques in T. hemsleyanum.

Key words: phytohormone metabolome, transcriptome, low-intensity blue light, Tetrastigma hemsleyanum, development of tuberous root, correlation and comparative analysis

中图分类号: