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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (21): 64-75.doi: 10.11924/j.issn.1000-6850.casb2025-0479

• 生物育种前沿共性技术 • 上一篇    下一篇

不同繁殖力新疆山羊发情周期下丘脑差异表达分析

全凯丽1(), 郑文新2(), 吕雪峰2,3, 邢巍婷2,3, 刘建新1   

  1. 1 新疆农业大学动物科学学院，乌鲁木齐 830052
    2 新疆农业大学，乌鲁木齐 830052
    3 新疆畜牧科学院畜牧业质量标准研究所，乌鲁木齐 830011
  • 收稿日期:2025-06-16 修回日期:2025-07-16 出版日期:2025-07-25 发布日期:2025-08-05
  • 通讯作者:
    郑文新，男，1972年出生，新疆乌鲁木齐人，研究员，博士生导师，博士，研究方向：动物遗传育种与繁殖，E-mail：。
  • 作者简介:

    全凯丽，女，1996年出生，新疆克州人，硕士研究生，研究方向：动物遗传育种与繁殖。通信地址：830052 新疆乌鲁木齐市新疆农业大学动物科学学院，E-mail：。

  • 基金资助:
    新疆维吾尔自治区自然科学基金重点项目“整合基因组学与转录组学探究新疆山羊繁殖性能的遗传机理”(2022D01D47)

Differential Expression Analysis of Hypothalamus During Estrous Cycle in Xinjiang Goats with Different Fertility Levels

QUAN Kaili1(), ZHENG Wenxin2(), LV Xuefeng2,3, XING Weiting2,3, LIU Jianxin1   

  1. 1 College of Animal Science, Xinjiang Agricultural University, Urumqi 830052
    2 Xinjiang Agricultural University, Urumqi 830052
    3 Institute of Animal Husbandry Quality Standards, Xinjiang Academy of Animal Sciences, Urumqi 830011
  • Received:2025-06-16 Revised:2025-07-16 Published:2025-07-25 Online:2025-08-05

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摘要：

为挖掘与新疆山羊繁殖性状相关的差异表达基因，筛选出与山羊繁殖相关的关键基因。本研究运用转录组测序分析法，比较新疆山羊不同繁殖力卵泡期和黄体期的下丘脑组织的转录组，筛选卵泡期和黄体期高、低繁殖力新疆山羊下丘脑与繁殖相关的关键基因。在卵泡期下丘脑差异表达分析中，XJLSX vs XJLDX组（卵泡期高繁殖力母羊vs卵泡期低繁殖力母羊）共筛选出680个差异表达基因，其中210个差异基因上调，470个下调，对差异表达基因进行GO和KEGG功能分析，发现了9个GO Term包含16个差异基因和8条KEGG通路包含23个差异基因与山羊繁殖性状相关，结合差异基因蛋白质互作网络分析及相关文献，共筛选出6个差异基因NPR1、KITLG、GABRAI、CRHR2、ADCYAP1、IGF2BP3，可能是影响新疆山羊繁殖性状的关键基因。在黄体期下丘脑差异表达分析中，XJHSX vs XJHDX组（黄体期高繁殖力母羊vs黄体期低繁殖力母羊）共筛选出656个差异表达基因，其中178个差异基因上调，478个差异基因下调，对差异表达基因进行GO和KEGG功能分析发现了7个GO Term包含9个差异基因与卵母细胞生长和分化、卵泡发育以及生殖激素相关，6条KEGG信号通路包含10个差异基因与山羊繁殖性状相关，结合差异基因蛋白质互作网络分析及相关文献，共筛选出5个差异基因WT1、IGF1、ESR1、NR5A1及MET，可能是影响新疆山羊繁殖性状的关键基因。本研究筛选出与山羊繁殖相关关键基因，这为培育新疆山羊高繁多胎新品系提供理论基础和分子标记。

关键词: 新疆山羊, 繁殖性状, 差异分析, 转录组

Abstract:

The study aims to explore differentially expressed genes related to reproductive traits in Xinjiang goats and screen out key genes associated with goat reproduction. In this study, transcriptome sequencing analysis was used to compare the transcriptomes of hypothalamic tissues between Xinjiang goats with different fecundity during the follicular phase and luteal phase, aiming to screen key reproduction-related genes in the hypothalamus of Xinjiang goats with high and low fecundity during these two phases. In the differential expression analysis of the hypothalamus during the follicular phase, a total of 680 differentially expressed genes were identified in the XJLSX vs XJLDX group (high-fecundity ewes vs low-fecundity ewes in the follicular phase), among which 210 were up-regulated and 470 were down-regulated. GO and KEGG functional analyses of these differentially expressed genes revealed 9 GO Terms containing 16 differentially expressed genes and 8 KEGG pathways containing 23 differentially expressed genes that were related to goat reproductive traits. Combined with protein-protein interaction network analysis of differentially expressed genes and relevant literature, 6 differentially expressed genes, namely NPR1, KITLG, GABRAI, CRHR2, ADCYAP1, IGF2BP3, were screened out as potential key genes affecting the reproductive traits of Xinjiang goats. In the differential expression analysis of the hypothalamus during the luteal phase, a total of 656 differentially expressed genes were found in the XJHSX vs XJHDX group (high-fecundity ewes vs low-fecundity ewes in the luteal phase), with 178 up-regulated and 478 down-regulated. GO and KEGG functional analyses showed that 7 GO Terms, involving 9 differentially expressed genes, were associated with oocyte growth and differentiation, follicular development, and reproductive hormones; 6 KEGG signaling pathways, containing 10 differentially expressed genes, were related to goat reproductive traits. Through integration with protein-protein interaction network analysis and literature review, 5 differentially expressed genes (WT1, IGF1, ESR1, NR5A1, MET) were identified as potential key genes influencing the reproductive traits of Xinjiang goats. This study screened out key genes related to goat reproduction, which provided a theoretical basis and molecular markers for breeding new high-fecundity and multi-fetal strains of Xinjiang goats.

Key words: Xinjiang goats, reproductive traits, differential analysis, transcriptome