欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (6): 66-66.

所属专题： 园艺

• 目次 • 上一篇    下一篇

蚕豆种子离体萌发和快繁技术研究

屈巧玲,胡润田,康诗腾,许凌波,王亦菲,,陆瑞菊,   

  • 出版日期:2006-06-05 发布日期:2006-06-05

Study on invitro of Germination and Rapid Propagation of Vicia faba L.

Qu Qiaoling, Hu Runtian, Kang Shiteng, Xu Lingbo, Wang Yifei,, Lu Ruiju,   

  • Online:2006-06-05 Published:2006-06-05

PDF (PC)

626

摘要： 以蚕豆为研究对象，比较了种子处理对种子发芽率的影响、培养基中添加AC，Vc 和 PVP对抑制外植体褐化的效果以及培养基中添加MET、香蕉、椰汁和马铃薯对试管苗繁殖系数及玻璃苗的影响。结果表明发芽时保留种皮、接种时胚根向上对蚕豆种子的萌发有着至关重要的影响，用5.0mg/L GA3溶液浸种12h有利于种子萌发；培养基中添加0.5g/L AC能有效抑制外植体的褐化，并能使植株生长健壮；培养基中添加150mg/L椰汁能提高繁殖系数，有效抑制玻璃苗的发生。

关键词: 农业产业结构, 农业产业结构, 可持续发展, 优化调整

Abstract: Using Vicia faba as donor materials, the effects of seed–treatment on germination, supplementing AC, Vc and PVP to induction media on explant–browning and adding MET, banana, potato and coconut water to propagating media on propagating coefficient and bud–vitrifiation were investigated. The results showed that the frequency of germination were able to be increased by keeping seed vessel, culturing seeds with radicle up and pre-treating seeds in 5.0mg/L GA3 solution for 12h. The frequency of explant-browning was reduced by adding AC 0.5mg/L to induction media and the vigor of plantlets was promoted by the AC-adding. The propagating coefficient could be improved and the frequency of bud-vitrifration increased by adding 150mg/L coconut water.