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中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (6): 62-62.

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直接诱导不定芽的矮牵牛再生体系的建立

陶妹英,贾彩红,徐碧玉,金志强   

  • 出版日期:2006-06-05 发布日期:2006-06-05

Establishment of A Regeneration System of Petunia Hybrida

Tao Meiying, Jia Caihong, Xu Biyu, Jin Zhiqiang   

  • Online:2006-06-05 Published:2006-06-05

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摘要： 以矮牵牛的叶片作为外植体，研究了不同浓度的激素配比对叶片直接诱导不定芽、诱导愈伤组织分化不定芽及生根的影响。得到了最适的直接诱导不定芽培养基的激素配6-BA1.5mg/L+IBA0.5mg/L、诱导愈伤组织分化不定芽的培养基的激素配比6-BA1.5 mg/L+NAA0.3~0.5 mg/L及生根培养基IBA0.03~0.05 mg/L。并在时间上，从芽的长势、芽的生根情况进行比较，结果表明：直接诱导的不定芽比诱导愈伤组织分化的不定芽所需的时间短、长势好、生根快。

关键词: 稻－鸭共生, 稻－鸭共生, 水稻, 模式

Abstract: The effects of different combination of hormone concentration on buds induction directly from leaf and calli, and shoots rooting are studied. The results indicated that the medium with 6-BA1.5mg/L+IBA0.5mg/L was the most suitable for buds induction directly from leaf, the medium with 6-BA1.5mg/L+NAA0.3~0.5mg/L for buds induction from calli, and the medium with IBA0.03~0.05mg/L was suitable for rooting. In addition, the time of buds induction directly from leaf is shorter than those from calli.