欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 409-412.

• 农学 农业基础科学 • 上一篇    下一篇

龙眼SRAP反应体系的建立和优化

赵玉辉 郭印山 傅嘉欣 周佳 黄穗生 刘成明   

  • 收稿日期:2009-04-15 修回日期:2009-05-06 出版日期:2009-09-20 发布日期:2009-09-20
  • 基金资助:

    龙眼童期、成熟期性状的QTL定位

Establishment and Optimization of SRAP Reaction System in Longan

  • Received:2009-04-15 Revised:2009-05-06 Online:2009-09-20 Published:2009-09-20

PDF (PC)

584

摘要：

采用分步优化的方法对影响龙眼SRAP-PCR反应的模板DNA用量、Mg2+浓度、dNTP浓度、引物浓度、TagDNA聚合酶用量等进行了研究。确立了适合龙眼SRAP分析的反应体系，即体系总体积25μl，包含1×PCR Buffer ，Mg2+ 2.0mmol/L，dNTPs 0.5 mmol/L，引物0.3μmol/L，模板DNA 10ng， TaqDNA聚合酶1.5 U。结果表明,该体系能很好地满足龙眼基因组SRAP扩增的要求，SRAP标记应用于龙眼遗传研究是可行的。

关键词: 早香橘橙, 早香橘橙, 套袋, 时间, 色泽

Abstract:

To establish appropriate longan SRAP-PCR reaction system, factors that affected the amplified result of SRAP such as amounts of DNA and Taq DNA polymerase, concentrations of Mg2+, dNTP and primer were studied by staged optimization method. Reaction system suitable for SRAP analysis was established. The reaction mixture (25μl) contained, 1 ×PCR Buffer, 2.0mmol/L Mg2+, 0.5 mmol/L dNTPs, 0.3μmol/L primer, 10 ng DNA template, and 1.5 U Taq DNA polymerase. The results showed the reaction system could meet SRAP amplification requirement of longan genome, and SRAP marker for longan genetic study was feasible.