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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (18): 284-288.

• 植物保护 农药 • 上一篇    下一篇

短短芽孢杆菌JK-2（Brevibacillus brevis）胞外物质抗香蕉枯萎病菌的稳定性

黄素芳 肖荣凤 杨述省 朱育菁 刘 波   

  • 收稿日期:2010-03-23 修回日期:2010-06-18 出版日期:2011-09-20 发布日期:2011-09-20

Stability of extracellular components of antagonistic bacterium JK-2, Brevibacillus brevis, against Fusarium oxysporum f. sp. cubense

  • Received:2010-03-23 Revised:2010-06-18 Online:2011-09-20 Published:2011-09-20

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摘要： 在30℃，170 r?min-1的条件下，恒温振荡培养36 h采样制备生防菌短短芽孢杆菌（Brevibacillus brevis）JK-2胞外物质，以香蕉枯萎病菌为指示菌，对胞外物质的抑菌特性进行研究。结果表明：该胞外物质具有一定的热稳定性，60~80℃温育1 h保持88.5%以上活性，在100℃放置1 h后无抑菌活性；在酸性pH 3.0～5.0和碱性pH 9.0～11.0条件下，抑菌活性保持在77.2％以上，对光照、蛋白酶K、紫外照射均不敏感。其抑菌作用的最适温度为30℃，最适pH值为7.0。

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Abstract: The extracellular component of antagonistic bacterium JK-2, Brevibacillus brevis, was prepared under the conditions of 30℃, 170 r?min-1 and 36 h cultural time and its inhibition ability of JK-2 against Fusarium oxysporum f. sp. cubense was studied. The results showed that the antifungal activity of the extracellular component preformed a certain thermal stability, remaining more than 88.5% after treated with 60-80℃ for 1 h; but no antifungal effect was found after it was incubated under 100℃ for 1 h. The antifungal activity also remained above 77.2% at the ranges of pH 3.0 to 5.0 and pH 9.0 to 11.0 and was nonsensitive to protease K, light and ultraviolet irradiation. The optimum conditions for Jk-2 to inhibit F. oxysporum f. sp. cubense was 30 ℃ and pH 7.0.