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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (4): 120-124.

• 林学 园艺 园林 • 上一篇    下一篇

匍枝筋骨草再生体系构建

赵晓杰 迟德富 李晓灿   

  • 收稿日期:2010-08-16 修回日期:2010-10-22 出版日期:2011-02-10 发布日期:2011-02-10
  • 基金资助:

    林业公益性行业科研专项;黑龙江省科技攻关项目

Study on the Regeneration System of Ajugalobata

  • Received:2010-08-16 Revised:2010-10-22 Online:2011-02-10 Published:2011-02-10

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摘要：

为了提高匍枝筋骨草繁殖速度，在短期内获得大量试管苗，为种质资源的安全和有效保存提供技术指导。本研究以匍枝筋骨草为试验材料进行组培试验。用匍枝筋骨草叶片培养获得了组培苗，建立起了良好的扩繁体系。结果表明，采用匍枝筋骨草嫩叶为外植体，3% NaClO处理10 min可达到较好的消毒效果。诱导不定芽增值的最适培养基为MS+6-BA 1 mg/L，增殖率高；诱导愈伤的最适培养基为MS+6-BA 1 mg/L+2,4-D 0.4 mg/L，所得愈伤组织大而绿，长势良好；诱导生根的最适培养基是MS+IBA 0.4 mg/L，所得根系发达，植株健壮。本实验建立了完整的匍枝筋骨草植株再生体系，为匍枝筋骨草的大规模生产奠定了基础。

关键词: 杂交兰, 杂交兰, 原球茎, 增殖, 分化

Abstract:

In order to increase the propagation rate for mass-produced Ajuga lobata and provide the technical guidance for the safety of germplasm resources and effectively preserve, the tissue culture and rapid propagation of Ajuga lobata was studied. The regeneration system of Ajuga lobata was well established by leaf blade. The results summarized as fellow: With 10 min NaClO 3% it can achieve good results by using leaves as explants. MS +6-BA 1 mg/L was the optimum multiplication culture medium. It can get high proliferation rate. MS+6-BA 1mg/L+2,4-D 0.4 mg/L was the optimum callus culture medium. The callus was large and green. MS+IBA 0.4 mg/L was the optimum culture medium taking roots. The roots were flourishing and the plant was strong. Thus the regeneration system of Ajuga lobata was established and it provided a foundation for Ajuga lobata mass production.

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