关键词: 甘蓝型油菜, 甘蓝型油菜, 植物防御素, 克隆, 生物信息学分析

Abstract: Endophytic diazoteophic bacteria DENB20 belonged to Klebsiella Trevisan. In order to investigate the colonizing dynamics of DENB20 in different desert plant species, this strain was marked by Rif and Str and was studied its colonizing dynamics in different desert plant species. The results showed that the population of DENB20 grew continuously in the roots of Tamarix ramosissima after inoculation, but in the stems and leaves of Tamarix ramosissima, the population of DENB20 decreased gradually and could not be isolated after 50 d inoculation. Although the population decreased gradually in the roots, stems and leaves of Lycium ruthenicum, there was still a certain amount of strain after 80 d inoculation. The development trend of DENB20 in the root of Poacynum hendersonii was first decreasing then increasing, but the population of DENB20 decreased gradually in the stems and leaves and could not be isolated after 50 d inoculation. In conclution, endophytic diazoteophic bacteria DENB20 could colonize stably in the roots of Tamarix ramosissima, Lycium ruthenicum and Poacynum hendersonii, and the colonization ability of DENB 20 in roots was as follows: Poacynum hendersonii＞Tamarix ramosissima＞Lycium ruthenicum.