泛素化修饰及其在植物蛋白质组学中的研究进展

doi:10.11924/j.issn.1000-6850.casb2020-002

中国农学通报 ›› 2020, Vol. 36 ›› Issue (30): 75-81.doi: 10.11924/j.issn.1000-6850.casb2020-002

所属专题：生物技术

泛素化修饰及其在植物蛋白质组学中的研究进展

张嘉麟¹^,²(), 李海英¹^,²()

¹黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨 150500
²黑龙江大学生命科学学院,黑龙江省普通高校分子生物学重点实验室,哈尔滨 150080

收稿日期:2020-04-12 修回日期:2020-06-23 出版日期:2020-10-25 发布日期:2020-10-16
通讯作者: 李海英
作者简介:张嘉麟,男,1997年出生,黑龙江佳木斯人,硕士研究生,研究方向：植物分子生物学。通信地址：150080 黑龙江省哈尔滨市学府路74号黑龙江大学生命科学学院320室,Tel：18846437879,E-mail： zx8262889@163.com。
基金资助:
国家自然科学基金“甜菜M14品系抗氧化酶系统响应盐胁迫应答过程的研究”(31471552);黑龙江省高校创新团队建设计划项目“寒区植物重要基因资源的挖掘与种质创新”(2014TD004)

Ubiquitin Modification and Its Research Progress in Plant Proteomics

Zhang Jialin¹^,²(), Li Haiying¹^,²()

¹Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500
²Key Laboratory of Molecular Biology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080

Received:2020-04-12 Revised:2020-06-23 Online:2020-10-25 Published:2020-10-16
Contact: Li Haiying

摘要/Abstract

摘要：

泛素化修饰是真核生物中一种重要的蛋白质翻译后修饰,几乎参与调控细胞内所有重要的生命活动过程。近年来,随着泛素化蛋白质富集技术和质谱技术的不断发展,泛素化蛋白质组学研究取得了重大进展。目前泛素化蛋白质组学研究多集中在动物和酵母中,而在植物中开展的相对较少。为了总结植物中泛素化蛋白质组学的最新研究成果,本文对泛素化修饰、质谱法鉴定泛素化位点、泛素化蛋白的富集方法及泛素化蛋白质组学在植物光调节、激素处理和逆境胁迫条件下的应用研究进行综述,为泛素化修饰在植物领域的最新研究提供理论基础。

关键词: 泛素化修饰, 泛素化蛋白的富集, 质谱, 植物蛋白质组学

Abstract:

Ubiquitination modification is an important post-translational modification of proteins, which participates in the regulation of many important life activities in cells. In recent years, with the development of ubiquitinated proteins enrichment technology and mass spectrometry technology, great progress has been made in ubiquitination proteomics. At present, there are many ubiquitination proteomics studies in animals and yeasts, but few in plants. In order to summarize the latest research results of ubiquitination proteomics in plants, the ubiquitination modifications, the identification of ubiquitination sites by mass spectrometry, the enrichment methods of ubiquitination proteins and the application of ubiquitination proteomics in plant light regulation, hormone treatment and stress conditions were reviewed in this paper, which could provide the rationale for the latest research of ubiquitination modifications in plants.

Key words: ubiquitination modifications, ubiquitinated proteins enrichment, mass spectrometry, plant proteomics

中图分类号:

S184

张嘉麟, 李海英. 泛素化修饰及其在植物蛋白质组学中的研究进展[J]. 中国农学通报, 2020, 36(30): 75-81.

Zhang Jialin, Li Haiying. Ubiquitin Modification and Its Research Progress in Plant Proteomics[J]. Chinese Agricultural Science Bulletin, 2020, 36(30): 75-81.

图/表 3

参考文献 48

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蛋白质/ 肽段	UBD	特异性
NEMO	UBA	M1>K63 泛素链
TAB2	NZF	K63泛素链
Ubiquilin-1	UBA	所有泛素链
MultiDsk	UBA	所有泛素链
RAP80	双重UIM	K63泛素链
TRABID	NZF	K29和K33泛素链

蛋白质/ 肽段	UBD	特异性
NEMO	UBA	M1>K63 泛素链
TAB2	NZF	K63泛素链
Ubiquilin-1	UBA	所有泛素链
MultiDsk	UBA	所有泛素链
RAP80	双重UIM	K63泛素链
TRABID	NZF	K29和K33泛素链

植物材料		组织	处理条件	富集方法		结果		参考文献
拟南芥 (Arabidopsis thaliana L.)		7日龄的黄化拟南芥幼苗	红光照射	GST-UBA亲和介质法泛素偶联His标签法		照射前130种泛素化蛋白照射后302种泛素化蛋白		[42]
玉米(Zea mays L.)		7日龄玉米黄化幼苗	光照射	K-ε-GG抗体法		1053种泛素化蛋白 1926个泛素化位点 78个差异表达泛素化位点		[43]
矮牵牛 (Petunia hybrida Mitchell)	花冠		乙烯处理		K-ε-GG抗体法		284个下调蛋白 233个上调蛋白	[44]
非洲水稻 (Oryza glaberrima)	12日龄的非洲水稻幼苗		45℃ 高温处理		K-ε-GG抗体法		264个上调肽段 147个下调肽段	[45]
马铃薯四倍体栽培品种 (Solanum tuberosum L.)	块茎培育的组培苗		聚乙二醇处理模拟干旱胁迫		K-ε-GG抗体法		3个下调位点 22个上调位点	[46]
茶 (Camellia sinensis L.)	两年龄的茶		干旱处理		K-ε-GG抗体法		14个上调位点 123个下调位点	[47]
水稻(Oryza sativa L.)	7日龄水稻幼苗		几丁质处理		K-ε-GG抗体法		144个位点上调 167个位点下调	[48]
水稻(Oryza sativa L.)	7日龄水稻幼苗		flg22处理		K-ε-GG抗体法		151个为位点上调 179个位点下调	[48]

植物材料		组织	处理条件	富集方法		结果		参考文献
拟南芥 (Arabidopsis thaliana L.)		7日龄的黄化拟南芥幼苗	红光照射	GST-UBA亲和介质法泛素偶联His标签法		照射前130种泛素化蛋白照射后302种泛素化蛋白		[42]
玉米(Zea mays L.)		7日龄玉米黄化幼苗	光照射	K-ε-GG抗体法		1053种泛素化蛋白 1926个泛素化位点 78个差异表达泛素化位点		[43]
矮牵牛 (Petunia hybrida Mitchell)	花冠		乙烯处理		K-ε-GG抗体法		284个下调蛋白 233个上调蛋白	[44]
非洲水稻 (Oryza glaberrima)	12日龄的非洲水稻幼苗		45℃ 高温处理		K-ε-GG抗体法		264个上调肽段 147个下调肽段	[45]
马铃薯四倍体栽培品种 (Solanum tuberosum L.)	块茎培育的组培苗		聚乙二醇处理模拟干旱胁迫		K-ε-GG抗体法		3个下调位点 22个上调位点	[46]
茶 (Camellia sinensis L.)	两年龄的茶		干旱处理		K-ε-GG抗体法		14个上调位点 123个下调位点	[47]
水稻(Oryza sativa L.)	7日龄水稻幼苗		几丁质处理		K-ε-GG抗体法		144个位点上调 167个位点下调	[48]
水稻(Oryza sativa L.)	7日龄水稻幼苗		flg22处理		K-ε-GG抗体法		151个为位点上调 179个位点下调	[48]

泛素化修饰及其在植物蛋白质组学中的研究进展

Ubiquitin Modification and Its Research Progress in Plant Proteomics

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