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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (10): 52-57.doi: 10.11924/j.issn.1000-6850.casb2024-0457

• 林学·园艺·园林 • 上一篇    下一篇

多毛番茄‘LA1777’叶片离体培养及再生体系的建立

顾文睿1(), 候约1, 周允1, 何继红2, 王天池1, 曹雪1()   

  1. 1 临沂大学农林科学学院，山东临沂 276000
    2 上海壹佰米网络科技有限公司，上海 200120
  • 收稿日期:2024-07-10 修回日期:2025-01-15 出版日期:2025-04-05 发布日期:2025-04-03
  • 通讯作者:
    曹雪，女，1984年出生，山东泰安人，副教授，博士研究生，研究方向：蔬菜种质创新和逆境生理与分子生物学。通信地址：276000 山东省临沂市兰山区双岭路中段 临沂大学农林科学学院，Tel：0539-7258720，E-mail：。
  • 作者简介:

    顾文睿，女，2000年出生，山东聊城人，本科在读，研究方向：园艺植物种质创新。通信地址：276000 山东省临沂市兰山区双岭路中段 临沂大学农林科学学院，Tel：0539-7258720，E-mail：。

  • 基金资助:
    国家自然科学基金项目“番茄miR398启动子参与低温响应的调控机理分析”(31801868); 大学生创新创业训练计划资助项目“多毛番茄LA1777叶片离体培养及再生体系的建立”(X202410452465); 山东省自然科学基金项目“miR398调控番茄响应温度逆境胁迫机理研究”(ZR2018PC023)

In Vitro Culture and Regeneration System of Solanum habrochaites ‘LA1777’ Leaves

GU Wenrui1(), HOU Yue1, ZHOU Yun1, HE Jihong2, WANG Tianchi1, CAO Xue1()   

  1. 1 College of Agriculture and Forestry Science, Linyi University, Linyi, Shandong 276000
    2 Shanghai 100me Internet Technology Co., Ltd., Shanghai 200120
  • Received:2024-07-10 Revised:2025-01-15 Published:2025-04-05 Online:2025-04-03

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摘要：

为筛选出最适合多毛番茄‘LA1777’叶片离体快繁的最佳配比培养基，采用离体培养的方法对多毛番茄进行试验。以多毛番茄‘LA1777’叶片为材料，MS培养基为基本培养基，同时设置不同浓度的激素配比，观察并统计愈伤组织、不定芽、不定根的诱导率及其生长状况。结果表明，促进叶片愈伤组织形成的最佳培养基配方为MS+ 3 mg/L 6-BA+ 0.3 mg/L IAA，出愈率为95.83%；促进不定芽分化的最佳培养基为MS+ 3 mg/L 6-BA+ 0.3 mg/L IAA+ 3 mg/L AgNO3，不定芽诱导率为50.00%；促进生根的最佳培养基为MS+ 0.1 mg/L NAA，不定根诱导率为94.44%。该研究结果可为多毛番茄种质资源的保存利用、快速繁殖和遗传转化提供参考。

关键词: 多毛番茄, 叶片, 离体培养, 愈伤分化, 不定芽诱导, 生根培养, 植株再生

Abstract:

In order to screen out the best ratio medium for in vitro rapid propagation of Solanum habrochaites ‘LA1777’ leaves, the method of in vitro culture was used. In this study, the leaves of S. habrochaites ‘LA1777’ were used as materials, MS medium was used as the basic medium, and different hormone combinations were set up, the induction rate and growth status of callus, adventitious buds and adventitious roots were observed and counted separately. The results showed that the best medium formula for promoting leaf callus formation was MS+ 3 mg/L 6-BA+ 0.3 mg/L IAA, and the callus rate was 95.83%. The best medium for promoting adventitious bud differentiation was MS+ 3 mg/L 6-BA+ 0.3 mg/L IAA+ 3 mg/L AgNO3, and the induction rate of adventitious buds was 50.00%. The best medium for rooting culture was MS+ 0.1 mg/L NAA, and the adventitious root induction rate was 94.44%. This study provides reference for germplasm conservation, rapid propagation and genetic transformation of S. habrochaites.

Key words: Solanum habrochaites, leaves, in vitro culture, callus differentiation, adventitious bud induction, rooting culture, plant regeneration