红曲霉生产红曲色素液体培养基优化

doi:10.11924/j.issn.1000-6850.casb2024-0550

摘要/Abstract

摘要：

本研究旨在提高红曲色素色价，为红曲色素的广泛应用提供技术支持。通过单因素实验结合响应面法(RSM)对红曲霉液体发酵培养基进行优化。首先通过单因素实验筛选出葡萄糖、蛋白胨和磷酸二氢钾为关键影响因素；随后利用Plackett-Burman实验和最陡爬坡实验确定各因素最佳响应区间；最终通过Box-Behnken实验优化得到最优培养基配方：葡萄糖59.3 g/L、蛋白胨30.5 g/L、磷酸二氢钾0.8 g/L、硫酸镁1.0 g/L、硫酸锌0.1 g/L；在此条件下，红曲色素色价最高值为268.49 U/mL，是优化前的3.23倍。研究表明，响应面法可高效优化优化培养基，显著提高红曲色素产量，为其工业化应用提供理论依据。

关键词: 红曲霉, 红曲色素色价, 单因素实验, 响应面实验, 培养基优化, Plackett-Burman实验, 最陡爬坡实验, Box-Behnken实验

Abstract:

The aim of this study is to improve the chromatic value of Monascus pigment, providing technical support for its widespread applications. To achieve the aim, the liquid medium for Monascus pigments production by Monascus purpureus fermentation was optimized using single-factor experiments and response surface methodology (RSM). Plackett-Burman design and steepest ascent experiments were employed to identify three key factors significantly affecting chromatic value of Monascus pigment including glucose, peptone, and KH₂PO_4,and the maximum response intervals of these factors were determined. Subsequently, a Box-Behnken design was used to optimize the medium composition, resulting in the following optimal medium formulation: glucose 59.3 g/L, peptone 30.5 g/L, KH₂PO₄ 0.8 g/L, MgSO₄1.0 g/L, ZnSO₄ 0.1 g/L. Under such optimized medium, the chromatic value of Monascus pigment reached 268.49 U/mL, which was 3.23 times higher than that achieved before optimization. The results demonstrate that response surface methodology is a highly efficient tool for optimizing culture media and this optimization significantly improves the yield of Monascus pigment.

Key words: Monascus purpureus, the chromatic value of Monascus pigment, single factor experiment, response surface experiment, medium optimization, Plackett-Burman test, steepest climb test, Box-Behnken test

潘冬梅, 刘振学, 杨传伦, 王红霞, 苏士岗, 刘结磊, 马娜娜, 部丽群, 王建平, 张雪飞, 韩滨鹏, 成鲁南. 红曲霉生产红曲色素液体培养基优化[J]. 中国农学通报, 2025, 41(20): 67-73.

PAN Dongmei, LIU Zhenxue, YANG Chuanlun, WANG Hongxia, SU Shigang, LIU Jielei, MA Nana, BU Liqun, WANG Jianping, ZHANG Xuefei, HAN Binpeng, CHENG Lunan. Optimization of Liquid Medium for Monascus Pigments Production by Monascus purpureus Fermentation[J]. Chinese Agricultural Science Bulletin, 2025, 41(20): 67-73.

图/表 15

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符号	因素/(g/L)	水平
符号	因素/(g/L)	-1	+1
A	葡萄糖	40.0	60.0
B	蛋白胨	20.0	30.0
C	磷酸二氢钾	0.8	1.2
D	硫酸镁	0.8	1.2
E	硫酸锌	0.08	0.12

符号	因素/(g/L)	水平
符号	因素/(g/L)	-1	+1
A	葡萄糖	40.0	60.0
B	蛋白胨	20.0	30.0
C	磷酸二氢钾	0.8	1.2
D	硫酸镁	0.8	1.2
E	硫酸锌	0.08	0.12

因素	水平/(g/L)
因素	-1	0	1
葡萄糖	55.0	60.0	65.0
蛋白胨	27.5	30.0	32.5
磷酸二氢钾	0.7	0.8	0.9

因素	水平/(g/L)
因素	-1	0	1
葡萄糖	55.0	60.0	65.0
蛋白胨	27.5	30.0	32.5
磷酸二氢钾	0.7	0.8	0.9

因素	平方和	自由度	均方	F值	P值	显著性
模型	6823.68	5	1364.74	20.04	0.0011	显著
A	734.30	1	734.30	10.78	0.0167	显著
B	4375.05	1	4375.05	64.25	0.0002	显著
C	1146.02	1	1146.02	16.83	0.0066	显著
D	472.38	1	472.38	6.94	0.0389	显著
E	95.94	1	95.94	1.41	0.2801	不显著