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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (27): 45-49.

所属专题： 水稻

• 农学 农业基础科学 • 上一篇    下一篇

水稻突变体的创制

罗洪发 查仁明 杨洪海 田永航 薛建蜂 张鹏   

  • 收稿日期:2010-12-20 修回日期:2011-05-30 出版日期:2011-10-25 发布日期:2011-10-25
  • 基金资助:

    “一个新水稻花器官突变体的分子定位研究”;利用EMS创造水稻新种质及其遗传多样性分析

Creation of Rice (Oryza sativa L.) Mutant

  • Received:2010-12-20 Revised:2011-05-30 Online:2011-10-25 Published:2011-10-25

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摘要：

为功能基因组学研究提供较好的基础材料，利用0.75%甲基磺酸乙酯溶液和γ射线双重诱变处理‘晋恢21’M1、‘中花11’、LR001种子进行研究。结果表明，经过M2筛选，获得716份叶、茎、穗、粒等性状变异的突变体，突变频率较高，达1.79%，还获得一些如不分蘖、多分蘖、白化苗、mini-plant、稀粒、花器官等极具研究价值的突变类型。双重诱变可以获得各种类型的突变体，所有的突变类型在3种材料中均有出现，说明该技术可为功能基因组研究提供大量的实物材料。

关键词: 江苏省, 江苏省

Abstract:

To provide materials for the research of rice functional genomics, dry seeds of ‘Zhonghua11’and LR001 were induced with 60Co γ-ray, then these seeds and ‘Jinhui21’ M1 seed, induced by ethyl methane sulfonate (EMS) of 1%, were induced with 1% EMS. Through the screening to M2, seven hundred and sixteen mutants, including the changed organ of leaf, stem, panicles and grain, etc, were identified. Among these ones, some were of great value in research, such as no-tillering, more-tillering, albino, mini-plant, thin grains, flower organs, etc. More types of mutants were obtained, with a frequencies of mutation mean of 1.79%, and all emerged in the three varieties in double induction. This indicated that the double mutation technology might provide a lot of mutants for functional genomics research.