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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (36): 24-30.doi: 10.11924/j.issn.1000-6850.casb16020013

所属专题： 水稻

• 农学 农业基础科学 • 上一篇    下一篇

不同类型江苏粳稻主推品种的遗传多样性分析

王 军1,2,宫丹妮3,范方军1,2,朱金燕1,2,李文奇1,2,王芳权1,2,仲维功1,2,杨 杰1,2   

  1. (1江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心,南京 210014；2扬州大学/江苏省粮食作物现代产业技术协同创新中心,江苏扬州 225009；3扬州大学生物科学与技术学院,江苏扬州 225009）
  • 收稿日期:2016-02-02 修回日期:2016-12-06 接受日期:2016-04-25 出版日期:2016-12-26 发布日期:2016-12-26
  • 通讯作者: 杨杰
  • 基金资助:
    公益性行业科研专项“粮食作物精确定量栽培技术研究与示范”(201303102)；江苏省现代农业重点研发项目“抗穗颈瘟、褐飞虱优良食味粳稻新品种选育”(BE2015355)；国家重大农技推广项目“洪泽县粮食作物新品种、新技术示范基地”(NG[15]003)。

Genetic Diversity Analysis of Major Japonica Cultivars of Different Ecotypes in Jiangsu

Wang Jun1,2, Gong Danni3, Fan Fangjun1,2, Zhu Jinyan1,2, Li Wenqi1,2, Wang Fangquan1,2, Zhong Weigong1,2, Yang Jie1,2   

  1. (1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014;2Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou Jiangsu 225009; 3College of Bioscience and Biotechnology, Yangzhou University, Yangzhou Jiangsu 225009)
  • Received:2016-02-02 Revised:2016-12-06 Accepted:2016-04-25 Online:2016-12-26 Published:2016-12-26

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摘要： 利用48对SSR分子标记对2007—2013年江苏省审定的65份不同类型常规粳稻品种和7份对照品种进行遗传多样性分析。结果表明：42对分子标记在供试材料间存在多态性，42对SSR标记共检测到101个等位基因，变化范围为2~4个，平均每个位点2.40个。基因多样性指数变异范围为0.03~0.64，平均0.21。多态信息含量(PIC)变异范围为0.03~0.58，平均0.18。65份江苏省常规粳稻品种间的遗传相似系数变异范围在0.78~0.97之间，平均0.91，95.4%的供试品种其遗传相似系数在0.87~0.97之间。3个不同类型的粳稻品种群体内，迟熟中粳检测出的等位基因数量最高，迟熟中粳的平均位点PIC值和基因多样性指数均高于晚粳品种和中熟中粳。UPGMA聚类结果表明，以遗传相似系数0.87为界，可将除‘通鉴981’、‘扬农稻1号’和‘盐稻10号’以外的62份江苏省常规粳稻品种分为2类；江苏省大面积生产主推粳稻品种的遗传多样性不够丰富，品种间的遗传距离较小，同一育种单位选育的品种间遗传距离更小。

关键词: 晚稻, 晚稻, 种子萌发期, 耐酸性, 比较

Abstract: Genetic diversity of 65 japonica rice varieties which were approved by Jiangsu from 2007 to 2013 and 7 control varieties were analyzed by 48 SSR primers. The results indicated that polymorphism existed in the experimental materials, and a total of 101 alleles loci were detected. The average number of alleles per SSR locus was 2.40 with a range from 2 to 4. The average value of gene diversity index was 0.21 with a range from 0.03 to 0.64. The value of polymorphism information content (PIC) was 0.18 with a range from 0.03 to 0.58. The intervarietal genetic similarity coefficient ranged from 0.78 to 0.97, with an average value of 0.91 for 65 japonica rice varieties. The genetic similarity coefficient ranged from 0.87 to 0.97 for 95.4% of the 65 japonica rice varieties. In three types of japonica varieties, genetic diversity of the late-maturation mid-season japonica had the highest number of allele, mean locus PIC value and genetic diversity index were higher than that of late japonica and mid-maturation mid-season japonica. Clustering analysis through UPGMA method showed that 65 japonica rice varieties could be clustered into two groups except ‘Tongjian 981’, ‘Yangnongdao1’ and ‘Yandao 10’. The japonica rice varieties in Jiangsu had close genetic relationship with each other and the genetic diversity was not enough at present. The varieties bred by the same breeding organization had a closer genetic relationship.