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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (14): 35-39.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

长白猪甘露聚糖结合凝集素-C基因启动子区的克隆与序列分析

刘玉芬   

  • 收稿日期:2011-01-10 修回日期:2011-01-28 出版日期:2011-06-15 发布日期:2011-06-15
  • 基金资助:

    哈尔滨师范大学青年学术骨干基金

Cloning and sequence analysis of mannan-binding lectin C promoter from Landrace

  • Received:2011-01-10 Revised:2011-01-28 Online:2011-06-15 Published:2011-06-15

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摘要：

摘要：根据GenBank上已发表的猪甘露聚糖结合凝集素C基因启动子区序列，设计一对特异性引物，通过RT-PCR法成功的从长白猪肝脏中克隆到MBL-C基因启动子区序列。扩增序列全长2030bp，与已发表的该序列相比具有3个SNPs位点，即-1636位点为T，-1614位点为A，-251位点为C，在-251位点与高水平表达的pMBL-C启动子区位点不同，从A突变为C。本研究为下一步分析pMBL-C的启动子区多态性奠定了基础。

关键词: 西葫芦, 西葫芦, 甜瓜, SSR, 通用性

Abstract:

Abstract: A pair of primers were designed according to the promoter region of from porcine mannose-binding lectin C published in GenBank, the promoter region of MBL-C from Landrace’s liver was acquired successfully by RT-PCR. There were 2030 bp in the sequence, three SNPs were found in the sequence,namely,at the position-1636 T,-1614A,-251C, it had one mutation at the position -251,from A to C. The study will provide base for analyzing promoter polymorphism of pMBL-C .