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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (12): 181-186.

所属专题： 生物技术； 园艺

• 农学 农业基础科学 • 上一篇    下一篇

橡胶树一个胶乳高表达MDR型ABC转运蛋白的克隆与表达研究

聂智毅 李波 白瑞璞 黎瑜   

  • 收稿日期:2011-01-24 修回日期:2011-02-18 出版日期:2011-05-25 发布日期:2011-05-25
  • 基金资助:

    国家自然科学基金;海南省自然科学基金

Cloning and Expression Analysis of a MDR-type ABC Transporter Abundant in Rubber Tree Latex

  • Received:2011-01-24 Revised:2011-02-18 Online:2011-05-25 Published:2011-05-25

PDF (PC)

1156

摘要：

ABC转运蛋白（ATP-Binding Cassette）是目前已知最大、功能最广泛的蛋白家族之一，与植物次生代谢物的排泌、积累及跨膜运转密切相关。本研究筛选已构建的乙烯刺激橡胶树胶乳消减文库，克隆了1个ABC转运蛋白基因EST，通过RACE技术获得其全长cDNA序列，该基因开放阅读框（ORF）共3753 bp，编码1251个氨基酸残基。研究表明其在橡胶树基因组中存在多个拷贝，主要在胶乳中表达，且在乙烯或茉莉酸刺激（刺激产胶）条件下上调表达。推测其可能与橡胶生物合成过程中的物质运输密切相关。

关键词: 剖面, 剖面

Abstract:

ABC (ATP-Binding Cassette) transporters constitute the largest protein family with the most variety of functions. They are involved in the excretion, accumulation and the transmembrane transport of plant secondary metabolites. Through screening an ethylene-induced latex SSH cDNA library combined with RACE, one ABC transporter gene EST and its full-length cDNA were obtained, which was 3753 bp with an ORF that encoded a polypeptide of 1250 amino acids. The results showed that the ABC transporter gene had multiple copies in rubber tree genome and was primarily expressed in latex. Under ethylene or jasmonic acid induction, its expression was up-regulated. Base on above analysis, we speculate that the ABC transporter is closely correlated to the transport of rubber biosynthesis metabolites.