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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (13): 212-215.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

芦笋单倍体染色体加倍技术研究

张天翔 林宗铿 蔡坤秀 杨俊杰   

  • 收稿日期:2011-02-16 修回日期:2011-03-14 出版日期:2011-06-05 发布日期:2011-06-05
  • 基金资助:

    福建省自然科学基金计划项目“利用游离小孢子培育芦笋超雄株技术的研究”

Research on the Technique of Chromosome Doubling of Haploids of Asparagus officinalis L.

  • Received:2011-02-16 Revised:2011-03-14 Online:2011-06-05 Published:2011-06-05

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摘要：

研究了芦笋单倍体染色体加倍的方法。在离体培养条件下，以秋水仙素为诱变剂，分别用浸泡法和培养基添加法处理芦笋单倍体幼苗的茎尖，比较了秋水仙素不同浓度、不同处理时间的诱导效果。结果表明，培养基添加法的诱导效果好于浸泡法，当在培养基中添加0.3%秋水仙素并处理7天时诱导效果最佳，染色体加倍频率与成活率分别可达82.50%和80%。加倍后的二倍体植株与单倍体植株相比，茎干变粗，气孔与保卫细胞增大，保卫细胞内的叶绿体数增多。

关键词: 硅, 硅, 镉, 水稻, 胁迫

Abstract:

The technique of chromosome doubling of haploids of Asparagus officinalis L. have been studied in this paper. In vitro chromosome doubling of haploids was studied by soaking shoot tips in colchicine solution and adding colchicine in the culture medium. The results indicated that adding colchicine in the culture medium increased the efficiency of chromosome doubling than that by soaking method. The induction rate and the survival rate of treating A. officinalis with 0.3% colchicine for 7 days by adding colchicine in the culture medium reached 82.50% and 80%. Compared with the haploid, the diploid stems showed thinker with larger guard cell and stoma and more chloroplasts.

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