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中国农学通报 ›› 2011, Vol. 27 ›› Issue (22): 46-50.

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

巴西橡胶树钙调蛋白基因的克隆及表达分析

陈鑫 朱家红 张治礼   

  • 收稿日期:2011-03-14 修回日期:2011-05-04 出版日期:2011-09-05 发布日期:2011-09-05
  • 基金资助:

    国家自然科学基金;中央级公益性科研院所专项基金;海南省自然基金

Cloning and Expression Analysis of Calmodulin Gene from Hevea brasiliensis

  • Received:2011-03-14 Revised:2011-05-04 Online:2011-09-05 Published:2011-09-05

摘要:

为了进一步研究橡胶生物合成的分子机制,根据巴西橡胶树(Hevea brasiliensis)cDNA文库中的EST序列,利用cDNA末端快速扩增(RACE)技术分离了一个巴西橡胶树钙调蛋白基因,命名为HbCAM1。分析结果显示,该基因cDNA全长775 bp,含有完整的阅读框架,编码区为450 bp,编码149个氨基酸,5’非编码区26 bp,3’非编码区299 bp。通过序列比对以及结构预测分析,HbCAM1编码的氨基酸序列与蓖麻、毛葡萄、烟草、麻风树中相应基因氨基酸序列的一致性分别达到100%、100%、99%、99%。半定量RT-PCR分析显示,HbCAM1基因可能通过对相关代谢基因表达调节参与了乙烯利刺激橡胶树增产的分子调控。

关键词: 长期试验, 长期试验, 稻-稻-紫云英轮作, 水稻产量, 杂草多样性

Abstract:

In order to clarify the processes and mechanisms of the biosynthesis of natural rubber, based on EST sequence from an SSH cDNA library of Hevea brasiliensis, a full-lengh cDNA encoding Calmodulin,designated HbCAM1,was cloned from H.brasiliensis by RACE-PCR, which has a total length of 775 bp with an open reading frame (ORF) of 450 bp and encodes 149 amino acid residues. The deduced amino acid sequence showed high identity of 100%,100%,99% and 99% to those of CAM from Ricinus communis,Vitis quinquangularis, Nicotiana attenuate and Jatropha curcas.Semi-quantitative RT-PCR analysis indicated that the transcription of HbCAM1 in latex was induced by tapping and ethphon treatment,suggesting that HbCAM1 may be involved in the regulation of ehphon-induced high latex yield in Hevea brasiliensis.