欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (12): 149-152.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

斜生褐孔菌液体发酵产物及菌核提取物的抑菌活性研究

王秋雯 王秋颖 邱文娜 唐丹   

  • 收稿日期:2011-03-14 修回日期:2011-03-30 出版日期:2011-05-25 发布日期:2011-05-25

Study on Antibacterial Activity of Extract of Submerged Fermentation Products and Sclerotium of Phaeoporus obliquus

  • Received:2011-03-14 Revised:2011-03-30 Online:2011-05-25 Published:2011-05-25

PDF (PC)

975

摘要：

研究并比较斜生褐孔菌发酵产物和菌核提取物的抑菌活性。采用石油醚、乙酸乙酯、正丁醇和水提取斜生褐孔菌发酵菌丝体和发酵液以及菌核中的有效成分，采用打孔法比较三个提取物中共10个组分的抑菌效果并测定了最低抑菌浓度（MIC）。结果表明斜生褐孔菌提取物对金黄色葡萄球菌和枯草芽孢杆菌具有抑制作用，且乙酸乙酯组分和正丁醇组分有明显的抑菌效果，水提取组分均无抑菌活性；斜生褐孔菌发酵菌丝体提取物的抑菌活性与菌核提取物的抑菌活性相当。该实验结果为更好的开发斜生褐孔菌发酵产物提供了理论基础。

关键词: 防治, 防治

Abstract:

The antibacterial activity of submerged fermentation products and sclerotium of Phaeoporus obliquus were investigated and compared. The effective components of submerged fermentation products and sclerotium of Phaeoporus obliquus was extracted with petroleum ether, ethyl acetate, butanol and water as the solvents resp. Perforated method was adopted to detect the antibacterial activity and the minimum inhibitory concentration (MIC) of 10 extra fractions. The result showed that the extra of Phaeoporus obliquus had inhibition on aureus staphylococcus and bacillus subtilis and the ethyl acetate fractions fractions and butanol fractions showed evident antibacterial effect, but all of the water fractions had no antibacterial effect. The antibacterial activity of the extra of mycelium was similar to the extra of Sclerotium. The study provided theoretical foundation for developing submerged fermentation products of Phaeoporus obliquus.