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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (22): 136-141.

• 林学 园艺 园林 • 上一篇    下一篇

马齿苋离体培养再生体系的建立

黄红梅 彭超宇 刘树玲 赵燕 刘清波   

  • 收稿日期:2011-03-21 修回日期:2011-06-10 出版日期:2011-09-05 发布日期:2011-09-05
  • 基金资助:

    湖南农业大学大学生创新性实验计划项目

Building-up of Regeneration System in vitro Culture of Portulaca oleracea

  • Received:2011-03-21 Revised:2011-06-10 Online:2011-09-05 Published:2011-09-05

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摘要：

以马齿苋幼嫩叶片和茎段为试验材料，对外植体灭菌、愈伤组织诱导、不定芽分化和试管苗生根的最佳条件进行研究，以期建立马齿苋再生技术体系，并为马齿苋的规模化繁殖和遗传改良等研究奠定基础。结果表明，最佳外植体灭菌方法为：用0.1%的升汞溶液浸泡8 min；最佳愈伤组织诱导培养基为MS+2,4-D 0.5 mg/L+6-BA 0.5 mg/L；最佳不定芽分化培养基为MS+6-BA 5.0 mg/L+NAA 0.4 mg/L；最佳生根培养基为1/2MS+IBA 2.0 mg/L。

关键词: 生理指标, 生理指标

Abstract:

To establish the regeneration technique system of Portulaca oleracea which lay the foundation for the research of large-scale propagation and genetic improvement, the optimal conditions for disinfecting of explants, inducing of callus, differentiating of adventitious buds and rooting of test-tube seedlings of Portulaca oleracea were studied by using leaf and stem bearing buds as explants. The results showed that the optimum disinfection method was to put explants in 0.1% HgCl2 for 8 min; MS+2,4-D 0.5 mg/L+6-BA 0.5 mg/L was the best medium to induce callus, MS+6-BA 5.0 mg/L+NAA 0.4 mg/L was the best sprout medium; The optimal culture medium was 1/2MS+IBA 2.0 mg/L for rooting.