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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (25): 158-161.

所属专题： 生物技术； 食用菌； 食用菌

• 林学 园艺 园林 • 上一篇    下一篇

黑木耳漆酶酶学性质的研究

肖楚 刘佳 许修宏   

  • 收稿日期:2011-05-04 修回日期:2011-06-17 出版日期:2011-10-05 发布日期:2011-10-05
  • 基金资助:

    科技部十一五科技支撑项目;科技部林业公益性行业科研专项;黑龙江省科技厅计划项目;哈尔滨市科技攻关计划

Studies on Enzymatic Properties of Laccase from auricularia

  • Received:2011-05-04 Revised:2011-06-17 Online:2011-10-05 Published:2011-10-05

PDF (PC)

861

摘要：

本实验从黑木耳液体发酵液中提取漆酶，对其酶学性质进行研究，为该漆酶的应用提供有价值的理论数据。对漆酶发酵液进行硫酸铵分级沉淀和透析，制得粗酶液。采用分光光度法，以ABTS[2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)]为底物测定漆酶活力。结果表明：黑木耳漆酶在55℃以下保温，漆酶活力和热稳定性良好。最适温度为45℃。温度超过65℃，出现明显的抑制作用。黑木耳漆酶最适pH为4.0，pH高于6.5，出现明显的抑制作用。金属离子对酶活表现出促进或抑制的作用。

关键词: 畜牧业, 畜牧业, 低碳经济, 污染, 资源, 节粮

Abstract:

Collecting laccase from the fermentation liquid of auricularia and studying the enzymatic properties in the experiment can provide worthful theory data for application of laccase. Laccase liquid is prepared by (NH4)2SO4 fractional precipitation and dialysis. By using spectrophotometry and choosing ABTS [2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)], the activity of laccase can be determined. The results showed that when the laccase was insulated below 55℃, the relative activity and thermal stability was good. The optimal temperature was 45℃. It could be inhibited when the temperature was above 65℃. The optimal pH was 4.0. It could be inhibited when the pH was above 6.5. Metal ions could inhibit or enhance the activity of laccase.

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