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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (25): 162-167.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

糖诱导桃果实园片果实苯丙氨酸解氨酶活性上升和酚类物质积累的研究

邓科 孔维府 战吉宬 王秀芹 黄卫东   

  • 收稿日期:2011-04-07 修回日期:2011-04-25 出版日期:2011-10-05 发布日期:2011-10-05
  • 基金资助:

    国家自然科学基金

The Study on Increasing Activity of Phenylalanine ammonia-lyase and Phenols Accumulation Induced by Sugar in Peach Fruit

  • Received:2011-04-07 Revised:2011-04-25 Online:2011-10-05 Published:2011-10-05

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摘要：

为了探讨OPPP途径与苯丙烷类代谢途径的关系，使用G6PDH的底物和产物以及蔗糖温育分别处理桃果肉圆片，测定了苯丙烷类代谢的关键酶——苯丙氨酸解氨酶的活性变化，以及酚类物质含量的变化。试验结果显示，葡萄糖-6-磷酸、6-磷酸葡萄糖酸、葡萄糖和蔗糖都可以提高PAL活性，相应的总酚和总类黄酮含量也明显增加。而葡萄糖的类似物，2-脱氧葡萄糖的效应和对照相似，对PAL活性和总酚、总类黄酮的含量影响很小。

关键词: 栽培, 栽培

Abstract:

In order to evaluate the direct relationship between G6PDH and phenylpropanoid metabolism, several kinds of sugars, including products of G6PDH, 6-phosphategluconate and sucrose were tested as to whether they enhance activity of phenylalanine ammonia-lyase and total flavonoids production in the tissue incubation system. The results indicated that the activity of PAL in fruit with sucrose, glucose, glucose-6-phosphate and 6-phosphategluconate incubation increased, and in concert on accumulation of total phenols and total flavonoids. However, 2-deoxyglucose and mannitol, which were inefficiently metabolized, had no effect on PAL activity and amount of phenols.