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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (3): 253-256.doi: 10.11924/j.issn.1000-6850.2011-1534

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

植物次生物质对棉蚜谷胱甘肽S-转移酶和羧酸酯酶活性的诱导作用

吕敏 孙婳婳 王丽红 高希武   

  • 收稿日期:2011-05-20 修回日期:2011-09-05 出版日期:2012-01-25 发布日期:2012-01-25

Effects of Secondary Metabolites on Activities of Glutathione S-transferases, Carboxylesterase in Aphid

  • Received:2011-05-20 Revised:2011-09-05 Online:2012-01-25 Published:2012-01-25

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摘要：

为了明确植物次生物质对昆虫解毒酶的影响作用，利用饲喂法和2-十三烷酮熏蒸法研究了植物次生物质2-十三烷酮和槲皮素对棉蚜谷胱甘肽S-转移酶和羧酸酯酶的诱导作用。结果表明取食含0.1% 2-十三烷酮饲料的棉蚜谷胱甘肽S-转移酶的活性最大，取食含0.01%槲皮素的饲料棉蚜羧酸酯酶的活性最大，均与对照差异显著。用2-十三烷酮熏蒸处理24 h，棉蚜谷胱甘肽S-转移酶的活性最大，与对照比差异显著。以α-NA和β-NA为底物测定棉蚜羧酸酯酶的活性在处理48 h达到最大，分别为对照的2.45和2.53倍，与对照比差异显著。由此可见，饲喂法处理2-十三烷酮对棉蚜谷胱甘肽S-转移酶及槲皮素对棉蚜羧酸酯酶的诱导作用具有显著的剂量效应；亚致死剂量2-十三烷酮熏蒸对棉蚜谷胱甘肽S-转移酶和羧酸酯酶的诱导作用具有显著的时间效应。

关键词: 黑龙江, 黑龙江, 农民工, 工资现状, 工资期望

Abstract:

The aim was to clarify the effects of plant allelochemicals on the detoxification enzyme activities in aphid. The effects of 2-tridecanone and quercetin on the specific activity of glutathione S-transferases (GSTs) and carboxylesterase (CarE) in aphid were investigated by using feeding and fumigation treatments. The results showed that the aphids fed on artificial diet added 0.1% 2-tridecanone and 0.01% quercetin showed the maximum activities of GSTs and CarE, respectively. The maximum activity of GSTs was at 24 h and the difference was significant compared to the control. The maximum activities of CarE measured using α-NA and β-NA as substrates were at 48 h, and the difference were significant compared the control. The activities were increased by 2.45-fold and 2.53-fold respectively compared to the control. Thus, the activities of GSTs and CarE were induced on dosage significantly, by feeding 2-tridecanone and quercetin respectively, and on time significantly by 2-tridecanone fumigation.