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中国农学通报 ›› 2011, Vol. 27 ›› Issue (21): 225-228.

所属专题: 生物技术

• 生物技术科学 • 上一篇    下一篇

胎生蜥蜴消化道生长抑素免疫活性内分泌细胞的研究

刘志涛 李淑兰 高欣 刘鹏 赵文阁 夏玉国   

  • 收稿日期:2011-05-26 修回日期:2011-07-19 出版日期:2011-08-25 发布日期:2011-08-25
  • 基金资助:

    黑龙江北部胎生蜥蜴种群动态与栖息地保护

The Research of Somatostatin Immunoreactve Endocrine Cells in Digestive Tract of Lacerta vivipara

  • Received:2011-05-26 Revised:2011-07-19 Online:2011-08-25 Published:2011-08-25

摘要:

为研究胎生蜥蜴消化道内生长抑素的分泌对动物整体营养及代谢水平的影响,应用免疫酶标技术 (ABC)法和胃肠激素抗血清,对胎生蜥蜴(Lacerta vivipara)消化道生长抑素细胞进行免疫组织化学定位研究和形态学观察。结果显示,生长抑素(SS)细胞分布广泛,除食管和直肠未检测到,整个消化道中均有分布,胃体和幽门分布密度最高,回肠最少,总体来说在胎生蜥消化道中SS细胞的分布密度胃部较高而小肠部较低。SS细胞以圆形和锥体形为主,还有梭形和椭圆形,它们分布于消化道粘膜、消化上皮细胞基部和腺泡上皮之间。根据其内分泌细胞的结构形态可以认为胎生蜥蜴消化道内的内分泌细胞兼具内分泌与外分泌2种功能。胎生蜥蜴消化道SS细胞的分布型与其他内分泌细胞抑制协调相关与其取食方式、食物成分及生活环境相关。

关键词: 绿肥压青, 绿肥压青, 促腐剂, 稻田土壤, 土壤肥力, 产量

Abstract:

The distribution and density of endocrine cells in the digestive tract of Lacerta vivipara were studied by the method of ABC (avidin-biotin complex method) immunohitochemical technique with gut hormone antisera, to study Lacerta vivipara digestive tract of somatostatin on the role of animal nutrition and metabolism. The result indicated that Somatostatin cells distributed throughout the digestive tract from Cardiacus to ileum. Somatostatin cells were not detected in the oesophagus and rectum. The density of somatostatin cells was the highest in the pylorus, followed by stomach part, and they were rarely observed in small intestine. Somatostatin cells were mainly in round shape and shuttled shape. They widely lied between epithelial cells, between glandular epithelial cells and at the bottom of epithelia. The distribution density of endocrine cells was related to its feeding habit, food component and living environment. And morphologies of endocrine cells were conformable with the endocrine and exocrine functions.