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中国农学通报 ›› 2011, Vol. 27 ›› Issue (24): 86-92.

所属专题: 资源与环境

• 农学 农业基础科学 • 上一篇    下一篇

粤东地区橄榄种质资源遗传多样性的ISSR分析

杨培奎 庄东红 马瑞君 赵谦 杜虹   

  • 收稿日期:2011-05-31 修回日期:2011-07-13 出版日期:2011-09-25 发布日期:2011-09-25
  • 基金资助:

    粤东地区橄榄种质资源调查及种质资源库的建立

The ISSR Analysis of Genetic Diversity of Canarium album L. Germplasm Resources in Eastern Guangdong

  • Received:2011-05-31 Revised:2011-07-13 Online:2011-09-25 Published:2011-09-25

摘要:

该研究在于分析粤东地区橄榄种质资源的遗传多样性,从而为橄榄的保护和研究提供依据。利用ISSR分子标记对64份橄榄种质进行遗传多样性分析。共扩增得到128条谱带,其中多态性条带99条,多态性比率为77.34%。平均观察等位基因数为1.7734±0.4203、有效等位基因数1.4823±0.3888、Nei’s基因多样性0.2746±0.1975、Shannon’s信息指数0.4072±0.2721。64份种质的遗传相似性系数范围是0.5714~0.9379,平均遗传相似性系数为0.7793。聚类分析将64份种质分为5个类群,主坐标分析也将其分为5个类群。由结果可知,该地区橄榄种质总体遗传多样性水平较低,同时分子聚类分析结果与供试材料来源地无关。

关键词: 黄瓜, 黄瓜, 果实横径, AD模型, SSR, QTL

Abstract:

The purpose of the present research was to analysis the genetic diversity of Canarium album L. germplasm resources in eastern Guangdong, which provided reference for protect and research in Canarium album L. germplasm resources. In the present study, genetic diversity of 64 accessions Canarium album were investigated by using ISSR markers. A total of 128 bands were generated, of which 99 bands were polymorphic, the percentage of polymorphic loci was 77.34%. Population genetics analysis showed that the average observed number of alleles (Na), effective number of alleles (Ne), Nei’s gene diversity (H)and Shannon’s information index (I) were, 1.7734±0.4203, 1.4823±0.3888, 0.2746±0.1975, and 0.4072±0.2721 respectively. The genetic similarity coefficient among 64 accessions ranged from 0.5714 to 0.9379, and the average was 0.7793. 64 accessions were clustered into 5 groups by the method of clustering analysis, and 5 groups by the method of principal coordinate analysis. The result indicated that the genetic diversity of Canarium album germplasm was low in these areas, and the results of molecular clusters had no correlation with the geographical origin of accessions.

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