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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (24): 302-306.

• 植物保护 农药 • 上一篇    下一篇

一株魔芋软腐病拮抗菌的分离鉴定及其活性物质的研究

吴金平 刘晓燕 张静柏   

  • 收稿日期:2011-06-02 修回日期:2011-07-25 出版日期:2011-09-25 发布日期:2011-09-25
  • 基金资助:

    湖北省重点自然科学基金

Isolation and Identification of Antagonistic Bacterium for Soft Rot in Amorphophallus konjac and the Study on Its Bioactive Substances

  • Received:2011-06-02 Revised:2011-07-25 Online:2011-09-25 Published:2011-09-25

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摘要：

在分离魔芋软腐病病原菌的过程中，获得一株对魔芋软腐病病原菌具有较强抑制作用的菌株Wh2。通过形态观察、生理生化实验、16S rDNA同源性序列分析，HPLC-MS分析，鉴定该菌为解淀粉芽孢杆菌，该菌在18.92 min时的洗脱液具有较明显的抑菌效果，此时的紫外吸收峰波长为229.5 nm，拮抗物质分子量为425 Da。

关键词: 冬瓜, 冬瓜, 平衡施肥, 产量, 品质

Abstract:

In the process of separating the soft rot pathogen in konjac, Wh2 was got which strongly inhibited the soft rot pathogen. According to the morphology characteristics, physiology and biochemistry tests, the comparison of 16S rDNA sequence, and HPLC-MS analysis, the Wh2 was similar Bacillus amyloliquefaciens. MS showed the active fraction was at the retention time of 18.92 min and the corresponding ultraviolet absorption peak wavelength was 229.5 nm. The molecular mass was 425 Da.

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