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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (28): 163-166.

• 林学 园艺 园林 • 上一篇    下一篇

亚洲百合试管苗生根的研究

刘婕 刘雅莉 贾敏 王岩   

  • 收稿日期:2011-06-20 修回日期:2011-08-04 出版日期:2011-11-05 发布日期:2011-11-05
  • 基金资助:

    百合ACC合成酶反义基因构建及其遗传转化

Research on Rooting of Asian Lily Plantlets

  • Received:2011-06-20 Revised:2011-08-04 Online:2011-11-05 Published:2011-11-05

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摘要：

为了优化亚洲百合生根培养基配方，为亚洲百合转基因植株试管苗的生根移栽奠定一定的基础，以亚洲百合‘普利安娜’（Lilium Asiatic hybrid cv. ‘Pollyanna’）和‘普瑞头’（Lilium Asiatic hybrid cv. ‘Prato’）试管苗为材料，探讨5种不同激素的浓度组合对试管苗生根率、生根数量、最长根长的影响。结果表明：不同植物激素的组合和浓度对亚洲百合的生根有显著影响，且2个品种生根状况有一定的差异。在生根培养基为MS+2,4-D 1.0 mg/L+KT 0.1 mg/L+6-BA 1.0 mg/L+蔗糖30 g/L+琼脂7 g/L时，亚洲百合‘普利安娜’平均生根率达到98.73%，生根数量达到6.53条，最长根长为4 cm；生根培养基为：MS+NAA 1.0 mg/L+TDZ 0.1 mg/L+蔗糖30 g/L+琼脂7 g/L时，亚洲百合‘普瑞头’平均生根率达到92.73%，生根数量达到6.63条，最长根长为3.77 cm。最后得出：亚洲百合‘普利安娜’最适宜的生根培养基为：MS+2,4-D 1.0 mg/L+KT 0.1 mg/L+6-BA 1.0 mg/L+蔗糖30 g/L+琼脂7 g/L；亚洲百合‘普瑞头’的适宜生根培养基为：MS+NAA 1.0 mg/L+TDZ 0.1 mg/L+蔗糖30 g/L+琼脂7 g/L。

关键词: 玉米杂交种, 玉米杂交种, 产量性状, 穗位叶, 光合性状, 灰色关联度分析

Abstract:

In order to optimize the formulation of Asian lily rooting medium and lay a foundation for rooting and transplanting of Asian lily transgenic plants, Lilium Asiatic hybrid cv. ‘Pollyanna’ and Lilium Asiatic hybrid cv. ‘Prato’ test-tube seedlings were used to study the effect of combinations of five different hormone in different concentrations on rooting rate, root number, longest root length. The results showed that: a combination of different types and concentrations of plant hormones significantly affected Asiatic lily rooting, and the rooting status of the two species had some differences. When using the rooting medium: MS+2,4-D 1.0 mg/L+KT 0.1 mg/L+6-BA 1.0 mg/L+sucrose 30 g/L+ agar 7 g/L, the average rooting rate of Lilium Asiatic hybrid cv. ‘Pollyanna’ could reach 98.73%, the root number of them could reach 6.53 cm and the longest root length of them was 4 cm. When using the rooting medium: MS+NAA 1.0 mg/L+TDZ 0.1mg/L+sucrose 30 g/L+agar 7 g/L, the average rooting rate of Lilium Asiatic hybrid cv. ‘Prato’ could reach 92.73%, the root number of them could reach 6.63 cm and the longest root length of them was 3.77 cm. The most appropriate rooting medium of Lilium cv. ‘Pollyanna’ was: MS+2,4-D 1.0 mg/L+KT 0.1 mg/L+6-BA 1.0 mg/L+sucrose 30 g/L+ agar 7 g/L; the most suitable rooting medium for Lilium cv. ‘Prato’ was: MS+NAA 1.0 mg/L+TDZ 0.1 mg/L+sucrose 30 g/L+agar 7 g/L.