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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (27): 122-126.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

一株纤维素酶产生菌的产酶条件优化

魏志文 张梅梅 赵艳霞 郑维发   

  • 收稿日期:2011-06-23 修回日期:2011-08-22 出版日期:2011-10-25 发布日期:2011-10-25
  • 基金资助:

    徐州师范大学自然科学基金

Optimization of Cellulase Production by Aspergillus niger No.Q9

  • Received:2011-06-23 Revised:2011-08-22 Online:2011-10-25 Published:2011-10-25

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摘要：

为了提高黑曲霉菌No.Q9(Aspergillus niger)的纤维素酶活力，应用于纤维素资源的降解研究。利用单因素实验和正交实验筛选碳氮源，优化培养基主要成分和发酵条件。结果表明菌株Q9的最适碳源为麦秸粉与麦麸，最适氮源为硫酸铵；最适培养基为：麦秸粉2%，麦麸1.5%，硫酸铵1.2%，pH 5.5；最适发酵条件为：接种量5%，培养温度30℃，摇床转速170 r/min，发酵时间3天。以上述条件发酵，菌株Q9的羧甲基纤维素酶活力达到165.21 U/mL，比优化前的123.79 U/mL提高了33.46%。菌株Q9的羧甲基纤维素酶活力较高，可以通过诱变等实验手段来进一步提高其酶活力。

关键词: 柑橘, 柑橘, 愈伤组织, 畸形胚状体, 可溶性物质, 保护酶

Abstract:

The aim was to increase the cellulase activity of Aspergillus niger No.Q9, and apply it in the degradation of cellulose resources. Single factor and orthogonal experiments were applied to screen of carbon and nitrogen sources and optimize the fermentation medium and culture conditions of Aspergillus niger No.Q9. The results showed that the optimal carbon source was wheat straw powder and bran, and nitrogen source was (NH4)2SO4. The optimized medium contained 2.00% straw power, 1.50% bran and 1.20% (NH4)2SO4, with pH 5.5. The optimum culture conditions contained 5% inoculum, 170 r/min rotation speed, on 30℃and culturing 3 days. The cellulase activity was increased by 33.46 % from 123.79 to 165.21 U/mL. Aspergillus niger No.Q9 had higher CMCase activity, and its enzyme activity could be enhanced through mutation experiments.

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