关键词: 高品质陆地棉, 高品质陆地棉, 转Bt基因抗虫棉, 株型性状, 产量性状, 遗传分析

Abstract:

Abstract: To study the effect of arginine and glutamine on the proliferation of porcine intestinal epithelial cell and investigate the transformation between arginine and glutamine, the test added different doses of arginine and glutamine in the cultures of porcine intestinal epithelial cell. Measured cell proliferation, and mRNA expression of P5CS, NAGS, NOS gene in cells, and the content of glutamine, arginine, spermine, nitric oxide in the supernatants. The results are as follows: glutamine supplementation increased the mRNA expression of NAGS gene, increased the content of arginine in cells, promoted the proliferation of cells. Arginine supplementation reduced the mRNA expression of P5CS and NAGS gene, inhibitted the transformation from arginine to glutamine, and high-dose arginine supplementation significantly reduced the content of glutamine in the supernatant of cells. The combined supplementation of arginine and glutamine than alone had better effect on proliferation of porcine intestinal epithelial cell. These results suggested that glutamine transferred to arginine and partial instead of arginine to play a role in porcine intestinal epithelial cell but arginine did not transfer to glutamine. Key words: piglet;intestinal epithelial cell; arginine; glutamine