欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (27): 70-74.

所属专题： 小麦

• 农学 农业基础科学 • 上一篇    下一篇

小麦空间诱变抗寒性突变体的初步研究

李鹏 孙明柱 张凤云 高国强 邱登林 李新华   

  • 收稿日期:2011-06-27 修回日期:2011-09-21 出版日期:2011-10-25 发布日期:2011-10-25

A Wheat Cold Resistance Mutant Derived From Space Mutagenesis

  • Received:2011-06-27 Revised:2011-09-21 Online:2011-10-25 Published:2011-10-25

PDF (PC)

1046

摘要：

为了研究小麦抗寒性机理，发掘抗寒性相关基因，以空间诱变创制的‘邯6172’抗寒性突变体作为研究对象，利用SRAP标记对突变体和对照的DNA进行了比较分析。结果表明，共用了380对引物，有6对引物在对照和突变体间扩增出了稳定的差异片段，占所用引物总数的1.58%，表明对照和突变体在DNA水平上的差异很小。在对照和突变体间扩增出了6条稳定的差异片段，3个存在于突变体中，对6个片段进行同源性比对，发现片段Me3-Em7-Mt、Me4-Em11-CK、Me7-Em19-CK和Me6-Em9-Mt的核苷酸序列都与小麦3B染色体序列具有较高的相似性，这表明影响突变体抗寒能力的基因或调控序列很可能位于3B染色体序列上。

关键词: 农业建设项目, 农业建设项目, 环境影响评价, 污染, 环境保护

Abstract:

In order to study the mechanism of wheat cold resistance, and discover genes associated with mutant cold resistance, a cold resistance mutant, obtained by spaceflight mutagenesis on the seeds of wheat variety ‘Han6172’, and the DNA of cold resistance mutant and contrast ‘Han6172’ were compared by SRAP technique. 380 pairs of primers were screened, 6 pairs of them had polymorphisms between mutant and contrast, the rate was 1.58%, and this data indicated that there were no obvious DNA differences between mutant and contrast. Six specific fragments were obtained, 3 fragments of them were amplified in mutant. Homology analysis in GenBank showed that Me3-Em7-Mt, Me4-Em11-CK, Me7-Em19-CK and Me6-Em9-Mt all had homologous sequences with wheat chromosome 3B-specific BAC library, and this result indicated that the gene and regulator sequences associated with mutant cold resistance might locate on 3B chromosome.

中图分类号: