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中国农学通报 ›› 2011, Vol. 27 ›› Issue (30): 182-186.

所属专题: 生物技术

• 生物技术科学 • 上一篇    下一篇

T4噬菌体的组装、感染及细胞裂解

颜晨 逯凯 任慧英   

  • 收稿日期:2011-09-18 修回日期:2011-09-29 出版日期:2011-11-25 发布日期:2011-11-25
  • 基金资助:

    大肠杆菌多价噬菌体尾丝蛋白结构与功能研究

Assembly, Infection and Cell Lysis of Bacteriophage T4

  • Received:2011-09-18 Revised:2011-09-29 Online:2011-11-25 Published:2011-11-25

摘要:

为深入开发利用T4噬菌体展示系统及T4噬菌体的临床细菌病治疗,应用现代各种分子生物学手段,在蛋白分子和组成结构上对T4噬菌体颗粒的各个重要组成部分进行分析,例如头部、尾部和尾板,描述了其蛋白构成和各种影响因子,并着重介绍了在噬菌体装配中最为重要的DNA的包装。同时针对噬菌体T4的感染与超感染免疫性和感染所导致的细胞裂解和裂解抑制等方面,介绍了这些现象发生的分子机制和影响因素。以期对T4噬菌体的分子装配和侵染机制有更为深刻的了解。

关键词: 河北省, 河北省, 草食家畜, 粗饲料, 优质饲草, 树叶, 饲养容量

Abstract:

To further develop and utilize T4 phage display and phage therapy, and open the broader prospect for the progress in study of T4 phage, the important components of T4 phage particles was analyzed by molecular biology techniques, such as the head, the tail and the basement. The protein composition of these structure were confirmed. DNA packing which was an important part in the assembly of T4 phage particle was described in detail. Not only the phenomenon of infection and lysis, but also the superinfection exclusion and lysis inhibition in T4 life cycles was introduced with molecular mechanism and impact factor. The assembly and infection mechanism of T4 phage would be known much better by these research.