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中国农学通报 ›› 2012, Vol. 28 ›› Issue (6): 73-78.

所属专题: 生物技术 棉花

• 农学 农业基础科学 • 上一篇    下一篇

棉花苗期耐盐生理指标的筛选及综合评价

刘雅辉 王秀萍 张国新 鲁雪林 张亚丽   

  • 收稿日期:2011-09-24 修回日期:2011-10-26 出版日期:2012-02-25 发布日期:2012-02-25
  • 基金资助:

    河北农林科学院青年基金项目

Study on Selection of Physiological Indices for Salt Tolerance and Comprehensive Evaluation of Cotton During Seedling Stage

  • Received:2011-09-24 Revised:2011-10-26 Online:2012-02-25 Published:2012-02-25

摘要:

为了筛选与棉花耐盐密切相关的生理指标,为棉花苗期耐盐鉴定提供理论依据。以4个棉花品种为材料,通过塑料盒栽植试验,探讨了棉花幼苗在盐胁迫前和胁迫后5天、10天、15天时的叶片相对含水量、质膜透性、游离脯氨酸含量、丙二醛含量、K+、Na+及K+/Na+值等耐盐相关生理指标相对值的变化情况,利用方差分析、主成分分析和隶属函数分析等方法对数据进行统计分析。结果表明所有指标在胁迫后第5天开始即与胁迫前表现极显著差异;选取K+/Na+值、相对含水量和质膜透性3个主要的耐盐鉴定生理指标;4个棉花品种的耐盐性强弱排序为‘枝棉3号’>‘中棉所35’>‘鲁棉6号’>‘中棉所12’。胁迫后第5天为最佳鉴定时机,所选取的耐盐生理指标代表了所有指标的信息,且其评价结果与省地方标准《棉花耐盐性鉴定评价技术规范》中盐害指数的评价结果高度相关,因此所选生理指标可与隶属函数法相结合用于棉花苗期耐盐综合评价中。

关键词: 观赏南瓜, 观赏南瓜, 种质资源, 引进筛选, 创新利用

Abstract:

In order to select physiological indices closely related to salt tolerance of cotton and provide theoretical basis for salt tolerance identifying in cotton seedling stage. The changes of relative value of seven physiological indices on 0, 5, 10, 15 days after salt stress were studied in seeding through planting experiment in plastic box. Four cotton breeds were used as materials. The indices included the relative water content, membrane permeability, dissociative proline content, malondialdehyde content and K+/Na+ ratio. The datum were analyzed by means of variance analysis, principal components analysis and membership function method. There was significant difference on relative value of all indices between the treatment and the control on the fifth day after salt stress; three indices, K+/Na+ ratio, relative water content and membrane permeability, were selected, which had close relation to salt tolerance; the salt tolerance capability of four cotton breeds decreased in order of zhimian3, zhongmiansuo35, lumian6, zhongmiansuo12. The fifth day after salt stress is the best appraisal time, the selected indices can be represent all information from seven indices, and its evaluation results have a high correlation with the evaluation results of index of salt in technical standard of evaluation for Salt Tolerance on cotton, so they can be used for comprehensive evaluation of cotton in seeding.