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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (24): 22-26.

所属专题： 生物技术； 玉米

• 农学 农业基础科学 • 上一篇    下一篇

玉米不同氮素水平下产量与zmAspAT基因表达分析

刘瑞响 曹晓良 陶勇生 张祖新   

  • 收稿日期:2012-03-20 修回日期:2012-04-09 出版日期:2012-08-25 发布日期:2012-08-25
  • 基金资助:

    国家重点基础研究发展计划（973项目）;国家转基因专项面上项目

The analysis of maize grain yield and zmAspAT gene expression patterns under different nitrogen conditions

  • Received:2012-03-20 Revised:2012-04-09 Online:2012-08-25 Published:2012-08-25

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摘要：

研究不同氮素水平下导入系L18和Y478产量及相关性状的差异以及与zmAspAT基因表达模式的关系。考察L18和Y478在低氮和正常供氮条件下的产量及相关性状，利用实时定量PCR技术分析zmAspAT基因的表达变化。在正常供氮条件下L18的单穗产量比Y478高17.21%，显著增加，行粒数极显著增加，百粒重显著减小；低氮条件下，L18单穗粒重比轮回亲本Y478显著减少，达到20.64%，行粒数显著增加，百粒重极显著减小。在低氮胁迫条件下，zmAspAT基因在L18中下调表达，在Y478则上调表达，表达模式不同。L18是氮低效的导入系，由于zmAspAT基因在L18与Y478表达模式不同，导致了L18较低的氮素利用效率和产量，zmAspAT是重要的玉米氮利用效率基因。

关键词: 基质, 基质

Abstract:

The difference of yield and yield-related traits between L18 and Y478 under different nitrogen levels was analyzed to find out the relationships with the zmAspAT gene expression pattern. The yield and yield-related traits of L18 and Y478 were evaluated under low and normal nitrogen conditions. The expression profile of the zmAspAT gene was assayed by quantity Real-time PCR. Under normal nitrogen conditions, compared to Y478, the grain yield of L18 significantly increased by 17.21%, kernel number per row also increased significantly, one hundred kernel weight of L18 decreased. Whereas, under low nitrogen levels, the grain yield of L18 remarkably decreased about 20.64%, kernel number per row increased, one hundred kernel weight decreased significantly. The expression of zmAspAT in L18 was down-regulated under N deficiency conditions, but its expression in Y478 was significantly up-regulated during stress. The expression profile of zmAspAT gene in L18 was significantly different from their expression in Y478. The L18 line was a low nitrogen use efficiency introgression line. The different expression levels of the zmAspAT gene in L18 and Y478 under low N conditions led to a low nitrogen use efficiency and grain yield. zmAspAT was an important gene for maize nitrogen use efficiency.

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