关键词: 构建, 构建

Abstract:

In order to know the discipline of protein accumulation, distribution of protein subunits and variation of soluble protein relative concentration during the pod-setting of southern Spanish-type peanut, the Kjeldahl method and SDS-PAGE electrophoresis were used to analyze the peanut seed protein and subunits of peptide fractions. The relative concentration of peanut protein band had been estimated by Bio-Rad Image lab TM. The results showed that, Spanish-type peanut seed protein content increased dramatically after 40-50 d of the flower, then gentle rising. Protein accumulated with the seed development of whole process, and arrived the highest point in mature period. Protein growth curve was different, because unlike genotype lead to difference of protein accumulation speed and strength. The type of peanut protein species had the higher polymorphism, protein subunit composition of different between genotype. The number of bands of mature stage was the least. The soluble protein relative concentration was different in unlike genotypes. The total soluble protein relative concentration distribution in 0.01-4.59, the peak appeared in 70-80 d. Subunits relative concentration could be divided without missing and missing type, missing subunits have appeared early, medium, late and both early and late. The type of later missing and both early and late take part in peanut protein synthesis, then degrade and disappear in late stage. Two genotypes of peanut protein subunits relative to the concentration change parabola accumulation to 68 kDa strip, the remaining strip showed fluctuation accumulation.