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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (10): 144-149.doi: 10.11924/j.issn.1000-6850.2012-3176

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

‘黑珍珠’番茄植株再生体系的研究

许飞云 张赵男 侯雨辰 王英正 王龙 王珂 徐进 赵宇   

  • 收稿日期:2012-09-19 修回日期:2012-09-25 出版日期:2013-04-05 发布日期:2013-04-05
  • 基金资助:
    2011年山西省高等学校大学生创新创业项目

The Study of Efficient Plant Regeneration of Solanum lycopersicum ‘Black’

  • Received:2012-09-19 Revised:2012-09-25 Online:2013-04-05 Published:2013-04-05

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摘要： 为了研究‘黑珍珠’番茄植株再生，以‘黑珍珠’番茄幼嫩叶片为外植体诱导愈伤组织，通过愈伤组织诱导培养、愈伤组织分化培养、不定芽增殖培养、生根培养和试管苗移栽，建立高效快速的‘黑珍珠’番茄再生体系。结果表明：最适宜的诱导叶片愈伤组织的培养基为MS+ 1.0 mg/L 6-BA+ 0.1 mg/L NAA，叶片外植体愈伤组织诱导率最高可达98.2%；诱导出的愈伤组织在MS+ 1.5 mg/L 6-BA+ 0.2 mg/L IBA培养基上能很好的分化出不定芽；MS+4.0 mg/L KT+ 0.01 mg/L IBA 培养基可实现不定芽芽增殖；最适宜的生根培养基为1/2MS+ (0.05~0.08) mg/L NAA，试管苗移栽成活率达92%。

关键词: 微量元素, 微量元素

Abstract: Procedures for callus induction, shoot regeneration, multiplication of shoot and rooting from regenerated shoots of ‘Black’ tomato were optimized by the study of several factor. The highest callus induction frequency was obtained from leaf explants on MS medium containing 1.0 mg/L benzylaminopurine (6-BA) with 0.1 mg/L α-naphthaleneacetic acid (NAA). The formed callus was transferred to regeneration medium supplemented with 6-BA and IBA and the best hormonal treatment to shoot regeneration was the medium with 1.5 mg/L 6-BA and 0.2 mg/L 3-indolebutyric (IBA). The medium containing 4.0 mg/L kinetin (KT) and 0.01 mg/L IBA was found to be the optimal hormonal treatment for multiplication. Also the medium with 0.05-0.08 mg/L NAA was found to be the best treatment for rooting, transplanting survival rate was 92%.

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