关键词: 分子鉴定, 分子鉴定

Abstract: Objective: The paper focused on the relationship between the microspore development and the morphological characteristics of the buds and anthers of three Angelica dahurica new lines, in order to judge the microspore developmental stage directly based on the morphological characteristics of the buds and anthers. Methods: The phenol magenta was selected for staining observation to A. dahurica microspore, then the developmental cytology, the relevance between the microspore development and the length of flower stalk, the diameter of bud, the length of anther and the color of bud and anther had been studied. Results: Microspore of A. dahurica developed at five stages: tetrad, early uninucleate, late-uninucleate, binucleate,trinucleate and developed at five stages: tetrad, early uninucleate, late-uninucleate, binucleate, trinucleate and each stage demonstrated distinctive characteristics. Microspore development also had correlation to the morphological characteristics of buds and the color of anther of the three A. dahurica lines. Among them, CX08-2 had the longest flower stalk, the diameter bud and the length of anther，then B-2, B-6 had the shortest. The length of flower stalk, the diameter of bud and the length of anther of late-uninucleate period had no significant difference with the adjacent period. The color of petal, dehisce or not and the color of anther could be the direct reference for anther culture. Conclusion: The microspore development period of A. dahurica. could be identified according to the floral organ morphology, it provided the foundation for selecting the best optimal explants sampling time for anther culture.