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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (25): 102-107.doi: 10.11924/j.issn.1000-6850.2013-0998

• 林学 园艺 园林 • 上一篇    下一篇

罗田栗实致腐真菌的分离鉴定及拮抗菌筛选

华娟 李淋玲 程华 程水源   

  • 收稿日期:2013-04-08 修回日期:2013-05-23 出版日期:2013-09-05 发布日期:2013-09-05
  • 基金资助:
    2011中央财政林业科技推广示范项目;湖北省自然科学基金重点项目;湖北省教育厅高校产学研合作资助重点项目

Isolation, identification and Screening of Antagonistic Bacteria of the Pathogenic Fungi in Chinese chestnut from Luotian County

  • Received:2013-04-08 Revised:2013-05-23 Online:2013-09-05 Published:2013-09-05

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摘要： 采用组织分离法，从无症和显症的罗田板栗栗实中分离致腐真菌，再利用形态学特征与真菌ITS rDNA序列分析相结合的方法鉴定分离真菌。结果表明，分离真菌经鉴定分属11个种：匍枝根霉(Rhizopus stolonifer)、腐皮镰刀菌(Fusarium solani)、黑葡萄穗霉(Stachybotrys chartarum)、栗疫菌(Cryphonectria parasitica)、可可毛色二孢菌(Lasiodiplodia theobromae)、扩展青霉(Penicillium expansum)、黑曲霉(Aspergillus niger)、胶胞炭疽菌(Colletotrichum gloeosporioides)、罗伦隐球酵母(Cryptococcus laurentii)、浅黄隐球酵母(Cryptococcus luteolus)、白假丝酵母(Candida albicans)；其中栗疫菌(Cryphonectria parasitica)和扩展青霉(Penicillium expansum)的分离率最高。供筛的6株拮抗细菌的平板对峙实验表明：丁香假单孢杆菌和荧光假单胞杆菌对板栗致腐真菌有抑制作用，其中荧光假单孢杆菌对板栗主要致腐真菌ITS4和ITS6的抑制圈直径超过10mm。

关键词: 河南省, 河南省

Abstract: The pathogenic fungi isolated from the decayed and non-symptom seeds of Chinese chestnut in Luotian, Hubei, China, identified into 11 species by morphological characteristics and phylogenetic characteristics based on ITS rDNA sequences. The results showed that those pathogenic fungi were Rhizopus stolonifer、Fusarium solani、Stachybotrys chartarum、Cryphonectria parasitica、Lasiodiplodia theobromae、Penicillium expansum、Aspergillus niger、Colletotrichum gloeosporioides、Cryptococcus laurentii、Cryptococcus luteolus、Candida albicans; the isolating rates of Cryphonectria parasitica and Penicillium expansum were higer than other isolated pathogenic fungi. Tablet confrontation test of 6 antagonistic bacteria strains showed that pathogenic fungi of Pseudomonas syringae and Pseudomonas fluorescens had remarkable antimicrobial activities to pathogenic fungi isolated from Chinese chestnut, Pseudomonas fluorescens showed higher resistant to pathogenic fungi TIS4 and ITS6 than Pseudomonas syringae.