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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (12): 279-282.doi: 10.11924/j.issn.1000-6850.2013-2135

• 生物技术科学 • 上一篇    下一篇

一株脉孢菌的鉴定及其挥发性物质GC-MS分析

陈利军 宁万光 史洪中 田雪亮   

  • 收稿日期:2013-08-07 修回日期:2013-09-02 出版日期:2014-04-25 发布日期:2014-04-25
  • 基金资助:
    国家自然科学青年科学基金(31101418)；河南省教育厅自然科学研究计划项目“豫南地区虫生真菌棒束孢属(Isaria)的多样性及脂肪酸分类研究”(2011C210001)。

The Identification of A Strain Neurospora sp. and Its Chemical Component Analysis of Volatile Compounds

  • Received:2013-08-07 Revised:2013-09-02 Online:2014-04-25 Published:2014-04-25

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摘要： 脉孢菌(Neurospora spp.)是常见的霉菌，有气味，为了研究脉孢菌气味挥发性物质的化学组分，采用18S rDNA序列分析对脉孢菌菌株FH-1进行鉴定，并采用气质联用仪(GC-MS)对其挥发性物质组分进行分析。结果表明：脉孢菌菌株FH-1的18S rDNA序列（登录号：KF312458）与粗糙脉孢菌(N. crassa)（登录号：AY046271.1）相似度为99%，被鉴定为粗糙脉孢菌；GC-MS从FH-1挥发性物质中分离到13个组分，鉴定了其中的9个组分，占挥发性物质总量的93.035%；挥发性物质主要由酯类和醇类组成，含量最高的组分为1-辛烯-3-醇，其相对含量为80.663%。

关键词: 动态变化, 动态变化

Abstract: Neurospora spp., with odor, was common mould. In order to research the chemical component of volatile compounds from Neurospora spp. odor, 18S rDNA sequence analysis was used to identify Neurospora sp. strain FH-1 and GC-MS was used to analyze its chemical component of volatile compounds. The results showed: the 18S rDNA sequence of Neurospora sp. strain FH-1 (Accession no. KF312458) was 99% identical to that of N. crassa (Accession no. AY046271.1), and was identified as N. crassa; 13 ingredients had been found, of which 9 identified by GC-MS, accounting for 93.035% of the total volatile compounds; esters and alcohols were the main chemical components of volatile compounds, and 1-octen-3-ol with the highest content (relative content 80.663%) of them.