中国农学通报 ›› 2015, Vol. 31 ›› Issue (1): 239-246.doi: 10.11924/j.issn.1000-6850.2014-0018
所属专题: 生物技术
杨倩,王丹,常丽丽,孙勇,靳翔,王旭初
收稿日期:
2014-01-02
修回日期:
2015-01-12
接受日期:
2014-06-23
出版日期:
2015-03-18
发布日期:
2015-03-18
通讯作者:
杨倩
基金资助:
Received:
2014-01-02
Revised:
2015-01-12
Accepted:
2014-06-23
Online:
2015-03-18
Published:
2015-03-18
摘要: 随着蛋白质组学的快速发展,生物质谱技术在蛋白质组学中的应用不断深入,逐渐成为这一领域的核心技术。为系统揭示生物质谱技术在蛋白质组学中的具体应用。本文综述了生物质谱仪器的快速发展和其在蛋白质鉴定、翻译后修饰和蛋白质定量分析等方面的应用和研究进展。生物质谱技术的进步加快了蛋白质组学研究的进程,但样品的复杂性和庞大数据的处理是生物质谱技术面临的不可避免的挑战。相信随着生物质谱技术的不断完善和改进,必将会在蛋白质组学研究中发挥更加重要的作用。
杨倩,王丹,常丽丽,孙勇,靳翔,王旭初. 生物质谱技术研究进展及其在蛋白质组学中的应用[J]. 中国农学通报, 2015, 31(1): 239-246.
[1] Cho A, Normile D. Nobel prize in chemistry: Masteringmacromolecules[J]. Science,2002,298(5593):527-528. [2] Shevchenko A, Ole N J, et al. Linking genome and proteome bymass spectrometry: large-scale identification of yeast proteins fromtwo- dimensional gels[J]. Proc. Natl. Acad. Sci. U. S. A, 1996,93(25):14440-14445. [3] Gygi S P, Corthals G L, et al. Evaluation of two- dimensional gelelectrophoresis-based proteome analysis technololgy[J]. [4] Wells W W, G H Wang, et al. Comparative Study of ThreeProteomic Quantitative Methods, DIGE, cICAT, and iTRAQ, Using2D Gel- or LC-MALDI TOF/TOF[J]. Journal of Proteome research,2006,5(3):651-658. [5] Fenn J B, Mann M, Meng C K, et al. Electrospray ionization formass spectrometry of large biomolecules[J].Science,1989,246(4926):64-71. [6] Vestal M L, Juhasz P, Martin S A. Delayed extraction matrixassisted laser desorption time-of-flight mass spectrometry[J].RapidCommunications in Mass Spectrometry,1995,9(11):1044-1050. [7] Tisato F B, Cristina P, Marina F R. Contribution of electrospraymass spectrometry for the characterization, design, anddevelopment of nitrido technetium and rhenium heterocomplexes aspotential radiopharmaceuticals[J].Mass Spectrometry Reviews,2004,23(5):309-332. [8] 盛龙生,苏焕华,郭丹滨.色谱质谱联用技术[M].北京:化学工业出版社,2005:38-42. [9] Bachi A, Bonaldi T. Quantitative proteomics as a new piece of thesystems biology puzzle[J].Journal of Proteomics,2008,71(3):357-367. [10] 王勇为.LTQ-Orbitrao Velos 双分压线性阱和静电场轨道阱组合式高分辨质谱性能及应用[J].2010,5. [11] Edman P, Begg G. A protein sequenator[J].European Journal ofBiochemistry,1967,1(1):80-91. [12] Yates J R. Mass spectral analysis in proteomics[J].Annual Reviewof Biophysics and Biomolecular Structure,2004,33:297-316. [13] Mintz P J, Kim J, Do K A, et al. Fingerpringting the circulatingrepertoire cancer patients[J].Nature Biotechnology,2002,21:57-63. [14] Witze E S, Old W M, Resing K A, et al. Mapping protein posttranslational modifications with mass spectrometry[J].NatureMethods,2007,4(10):798-806. [15] Zolnierowicz S, Bollen M. Protein phosphorylation and proteinphosphatases De Panne, Belgium, September 19- 24,1999[J].TheEMBO Journal,2000,19(4):483-488. [16] Schulenberg B, Goodman T N, et al. Characterization of dynamicand steady- state protein phosphorylation using a fluorescentphosphoprotein gel stain and mass spectrometry [J]. Electrophoresis,2004,25(15):2526-2532. [17] Pandey A, Podtelejnikov A V, Blagoev B, et al. Analysis of receptorsignaling pathways by mass spectrometry: identification of vav-2 asa substrate of the epidermal and platelet- derived growth factorreceptors[J].Proc Natl Acad Sci, USA,2000,97(1):179-184. [18] Rush J, Moritz A, Lee KA, et al. Immunoaffinity profiling oftyrosine phosphorylation in cancer cells[J]. Nature Biotechnology,2005,23(1):94-101. [19] Gronborg M, Kristiansen T Z, Stensballe A, et al. A massspectrometry- based proteomic approach for identification of Serine/threonine- phosphorylated proteins by enrichment with phosphospecific antibodies: identification of a novel protein, frigg, as aprotein kinase a substrate[J].Molecular Cellular Proteomics,2002,1(7):517-527. [20] Larsen M R, Thingholm T E, Jensen O N, et al.Highly selectiveenrichment of phosphorylated peptides from peptide mixtures usingtitanium dioxide microcolumns[J].Molecular Cellular Proteomics,2005,4(7):873-886. [21] Wu J, Shakey Q, Liu W, et al. Global profiling of phosphopeptidesby Titania affinity enrichment[J].Journal of Proteome Research,2007,6(12):4684-4689. [22] 迟明,毕炜,卢庄,等.天冬氨酸作为非特异性吸附抑制剂在二氧化钛选择性富集磷酸肽中的应用[J].色谱,2010,28(2):152-157. [23] Li Q R, Ning Z B, Tang J S, et al. Effect of peptide-to-TiO2 beadsratio on phosphopeptide enrichment selectivity[J].Journal ofProteome Research,2009,8(11):5375-5381. [24] Kweon H K, Hakansson K. Selective zirconium dioxide- basedenrichment of phosphorylated peptides for mass spectrometricanalysis[J].Analytical Chemistry,2006,78(6):1743-1749. [25] Wolschin F, Wienkoop S, Weckwerth W. Enrichment ofphosphorylated proteins and peptides from complex mixtures usingmetal oxide/hydroxide affinity chromatography[J]. Proteomics,2005,5(17):4389-4397. [26] Posewitz M C, Tempst P. Immobilized gallium( Ⅲ) affinitychromatography of phosphopetides[J].Analytical Chemistry,1999,71(14): 2883-2892. [27] Stensballe A, Andersen S, Jensen O N. Characterization ofphosphpproteins from electrophoretic gels by nanoscale Fe( Ⅲ)affinity chromatography with off-line mass spectrometry analysis[J].Proteomics,2001,1(2):207-222. [28] Ficarro S B, McCleland M L, Stukenberg P T, et al.Phosphoproteome analysis by mass spectrometry and its applicationto Saccharomyces cerevisiae[J].Nature Biotechnology,2002,20(3):301-305. [29] Seeley E H, Riggs L D, Regnier F E. Reduction of non- specificbinding in Ga(Ⅲ) immobilized metal affinity chromatography forphosphopeptides by using endoproteinase glu- C as the digestiveenzyme[J].Journal of Chromatography B,2005,817(1):81-88. [30] Hart S R, Waterfield M D, Burlingame A L, et al. Factors goveringthe solubilization of phosphopeptides retained on ferric NTA IMACbeads and their analysis by MALDI TOFMS[J].Journal of theAmerican Society for Mass Spectrometry,2002,13(9):1042-1051. [31] Barnouin K N, Hart S R, Thompson A J, et al. Enhancedphosphopeptide isolation by Fe( Ⅲ)- IMAC using 1,1,1,3,3,3-hexafluoroisopropanol[J].Proteomics,2005,5(17):4376-4388. [32] Zhang Y H, Yu X J, Wang X Y, et al. Zeolite nanoparticles withimmobilized metal ions: isolation and MALDI- TOF- MS/MSidentification of phosphopeptides[J].Chemical Communications,2004,44(24): 2882-2883. [33] Oda Y, Nagasu T, Chait B T. Enrichment analysis of phosphorylatedproteins as a tool for probing the phosphoproteome[J]. NatureBiotechnology,2001,19:379-382. [34] Zhou H, Watts J D, Aebersold R. A systimatic approach to theanalysis of protein phosphorylation[J].Nature Biotechnology,2001,19:375-378. [35] Larsen M R, Sorensen G L, Fey S J, et al. Phospho- proteomics:evaluation of the use of enzymatic de- phosphorylation anddifferential mass spectrom etric peptide mass mapping for sitespecific phosphorylation assignment in proteins separated by gelelectrophoresis[J]. Proteomics,2001,1(2):223-238. [36] Ma Y, Lu Y, Zeng H, et al.Characterization of phosphopeptidesfrom protein digests using matrix- assisted laser desorption/ionization time- of- flight mass spectrometry and nanoelectrosprayquadrupole time- of- flight mass spectrometry[J].RapidCommunications in Mass Spectrometry,2001,15(18):1693-1700. [37] Amankwa L N, Harder K, Jirik F, et al. High- sensitivitydetermination of tyrosine- phosphorylated peptides by on- lineenzyme reactor and electrospray ionization mass spectrometry[J].Protein Science,1995,4(1):113-125. [38] Gruhler A, Olsen J V, Mohammed S, et al. Quantitaivephosphoproteomics applied to the yeast pheromone signalingpathway[J].Molecular Cellular Proteomics,2005,4(3):310-327. [39] Jacobs J M, Mottaz H M, Yu L R, et al. Multidimensional proteomeanalysis of human mammary epithelial cells[J].Journal of ProteomeResearch,2004,3(1):68-75. [40] Shen Y F, Jacobs J M, Camp D G, et al. Ultra- high- efficiencystrong cation exchange LC/RPLC/MS/MS for high dynamic rangecharacterization of the human plasa proteome[J].AnalyticalChemistry,2004,76(4):1134-1144. [41] Stensballe A, Jensen O N, Olsen J V, et al. Electron capturedissociation of singly and multiply phosphorylated peptides[J].Rapid Communications in Mass Spectrometry,2000,14(19):1793-1800. [42] Yang Y, Orlando R. Identifying the glycosylation sites andsitespecific carbohydrate heterogeneity of glycoproteins by matrixassisted laser desorption/ionization mass spectrometry[J].RapidCommunications in Mass Spectrometry,1996,10(8):932-936. [43] Harmon B J, Gu X J, Wang D C. Rapid monitoring of site-specificglycosylation miroheterogeneity of recombinant human interferon-γ[J].Analytical Chemistry,1996,68(9):1465-1473. [44] Chen R, Jiang X N, Sun D G, et al. Glycoproteomics analysis ofhuman liver tissue by combination of multiple enzyme digestionand hydrazide chemistry[J].Journal of Proteomics Research,2009,8(2):651-661. [45] Tsarbopoulos A, Pramanik B N, Nagabhushan T L, et al. Structural analysis of the CHO- derived interleukin- 4 by liquidchromatography/electrospray ionization mass spectrometry[J].Journal of Mass Spectrometry,1995,30(12):1752-1763. [46] Yan W, Chen S S. Mass spectrometry-based quantitative proteomic profiling[J].Briefing in Functional Genomics and Proteomics,2005,4(1):27-38. [47] Larance M, Bailly A P, Pourkarimi E, et al. Stable-isotope labelingwith amino acids in nematodes[J].Nature Methods,2011,8(10):849-851. [48] Zhao Y, Jia W, Sun W, et al. Combination of improved 18Oincorporation and multiple reaction monitoring: a universal strategy for absolute quantitative verification of serum candidate biomarkersof liver cancer[J].Journal of Proteomics Research,2010,9(6):3319-3327. [49] Shiio Y, Aebersold R. Quantitative proteomic analysis using isotopecoded affinity tags and mass spectrometry[J].Nature Protocol,2006,1(1):139-145. [50] Zhu M M, Dai S J, et al. Functional differentiation of Brassicanapus guard cells and mesophyll cells revealed by comparativeproteomics[J]. Molecular Celluar Proteomics, 2009,8(4):752-766[51] Wang W X, Zhou H H, Lin H, et al. Quantification of proteins and metabolites by mass spectrometry without isotopic labeling orspiked standards[J].Analytical Chemistry,2003,75(18):4818-4826. [51] Wang W X, Zhou H H, Lin H, et al. Quantification of proteins and metabolites by mass spectrometry without isotopic labeling or spiked standards[J].Analytical Chemistry,2003,75(18):4818-4826. |
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