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中国农学通报 ›› 2015, Vol. 31 ›› Issue (9): 216-223.doi: 10.11924/j.issn.1000-6850.casb15010125

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

微生物菌肥作用下荒漠土壤微生物群落结构和功能研究

吴江利,罗学刚   

  1. 西南科技大学,西南科技大学
  • 收稿日期:2015-01-19 修回日期:2015-03-10 接受日期:2015-02-09 出版日期:2015-04-07 发布日期:2015-04-07
  • 通讯作者: 罗学刚
  • 基金资助:
    国家农业科技成果转化资金项目(2012GB2G200469),西南科技大学研究生创新基金资助项目 (14ycx071)

Researches on Microbial Community Structure and Function in Desert Soil under Microbial Fertilizer

  • Received:2015-01-19 Revised:2015-03-10 Accepted:2015-02-09 Online:2015-04-07 Published:2015-04-07

摘要: 为了探究施用微滴灌生物菌肥后对土壤的影响,笔者以经过微滴灌菌肥处理、种植棉花的荒漠土壤为研究对象,采用常规的稀释平板法和Biolog-Eco微平板反应系统研究了4种不同浓度的微生物菌剂(A、R、G、K)对荒漠土壤微生物群落结构及功能多样性的影响。结果表明,各处理组的土壤中的微生物数量关系为:细菌>真菌>放线菌;相比对照组,处理组AWCD提高了3.7%~25.6%,并且AWCD、Shannon指数(H’)、Simpson指数(D)与有机质、全氮、可溶性磷、全钾等的含量呈现显著的正相关;不同种类的微生物菌肥影响了不同土壤中微生物群落间的代谢特征,通过主成分分析表明碳水化合物类碳源是这种变化重要指示指标。本试验结果可为微滴灌微生物肥料的使用效果以及荒漠土壤微生物群落结构评价提供一定的参考。

关键词: 木薯渣, 木薯渣, 肉鸭养殖, 生长性能, 成活率, 生产环境, 经济效益

Abstract: In order to explore the influence of micro drip bio-bacterial manure on soil, the author took desert soil as example, which had been treated with micro drip bio- bacterial manure dispose and after cotton cultivation, to conduct the research. The effects of microbial agents with 4 different concentrations (A, R, G and K) were studied through conventional dilution- plate method and Biolog- Eco system, and its effects on the microbial community structure and functional diversity were analyzed. The results showed that the order of microorganisms in different treatments was bacteria>fungi>actinomycete. AWCD increased 3.7% to 25.6%. Different bio- bacterial manure influenced the characteristics of different soil microbial communities differently. Principal component analysis (PCA) showed that carbon source of carbohydrate was an important index of this variation. The results could provide certain references for the application of micro drip biobacterial manure and the evaluation of microbial community structure in desert soil.