苹果腐烂病菌侵染过程中枝条相关病程物质含量的变化

doi:10.11924/j.issn.1000-6850.casb15050148

中国农学通报 ›› 2015, Vol. 31 ›› Issue (34): 177-186.doi: 10.11924/j.issn.1000-6850.casb15050148

所属专题：园艺

苹果腐烂病菌侵染过程中枝条相关病程物质含量的变化

刘斐,谭贻,王忆,吴婷,张新忠

(中国农业大学农学与生物技术学院果树系,北京 100193）

收稿日期:2015-05-25 修回日期:2015-08-05 接受日期:2015-08-25 出版日期:2015-12-17 发布日期:2015-12-17
通讯作者: 张新忠
基金资助:
公益性行业（农业）科研专项“我国重要野生果树资源的收集、评价与优异种质创新利用技术研究与示范”(201303093)；国家863课题“苹果分子育种与种质创制”(2011AA001204)。

Change of Disease Related Substances Content in Branches During the Process of Valsa ceratosperma.

Liu Fei, Tan Yi, Wang Yi, Wu Ting, Zhang Xinzhong

(College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193)

Received:2015-05-25 Revised:2015-08-05 Accepted:2015-08-25 Online:2015-12-17 Published:2015-12-17

摘要/Abstract

摘要： 为探究苹果腐烂病的抗病机制，分别用中抗腐烂病种质‘法8’和‘高5’，感病种质‘紫云’和‘红富士’以及高感种质‘嘎拉’和‘金冠’的1年生休眠枝离体接种腐烂病菌株03-8的菌丝，接种后0~7天检测接种部位周围皮层健康组织中病程相关物质多酚、水杨酸和木质素含量变化。结果表明：所有种质接种后1~7天皮层中对香豆酸、肉桂酸和绿原酸含量均显著升高，但仅绿原酸含量与腐烂病的病斑长度显著负相关(r=-0.906)。接菌后皮层组织水杨酸和木质素含量显著增加，水杨酸和木质素含量与病斑长度显著负相关(r=-0.825；r=-0.948)。因此，接种苹果腐烂病菌后皮层水杨酸、木质素和绿原酸含量的显著增加与苹果抗腐烂病相关。

关键词: 淮河流域, 淮河流域, 夏玉米, 作物需水量, 有效降水量, 水分盈亏指数

Abstract: To elucidate the physiological mechanism of the resistance to apple canker, one-year-old apple branches of the moderately resistant accessions ‘French 8’ and ‘Gao 5’, the susceptible cultivars ‘Whitney’ and ‘Red Fuji’ and highly susceptible cultivars ‘Gala’ and ‘Golden delicious’ were inoculated in vitro with mycelia of Valsa ceratosperma isolate 03-8. Zero to seven days after the inoculation, the changes in polyphenols, salicylic acid and lignin were detected. The results indicated that 0-7 days after inoculation, p-coumaric acid, cinnamic acid and chlorogenic acid increased significantly, but only the content of chlorogenic acid was negatively correlated with Valsa canker lesion length (r=-0.906). After inoculation with mycelia, salicylic acid and lignin contents in the cortical tissue significantly increased, the content of salicylic acid and lignin was significantly and negatively correlated with Valsa canker lesion length (r=-0.825; r=-0.948). Therefore, the increase of salicylic acid, lignin and chlorogenic acid in apple was correlated with the resistance to Valsa ceratosperma.

中图分类号:

S661.1

刘斐,谭贻,王忆,吴婷,张新忠. 苹果腐烂病菌侵染过程中枝条相关病程物质含量的变化[J]. 中国农学通报, 2015, 31(34): 177-186.

Liu Fei,Tan Yi,Wang Yi,Wu Ting and Zhang Xinzhong. Change of Disease Related Substances Content in Branches During the Process of Valsa ceratosperma.[J]. Chinese Agricultural Science Bulletin, 2015, 31(34): 177-186.

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苹果腐烂病菌侵染过程中枝条相关病程物质含量的变化

Change of Disease Related Substances Content in Branches During the Process of Valsa ceratosperma.

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