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中国农学通报 ›› 2016, Vol. 32 ›› Issue (14): 32-36.doi: 10.11924/j.issn.1000-6850.casb15110157

所属专题: 生物技术

• 水产 渔业 • 上一篇    下一篇

东平湖鲤染色体组型分析

卢 红,孟庆磊,杨 玲   

  1. (山东省淡水渔业研究院,山东省淡水水产遗传育种重点实验室,济南 250013)
  • 收稿日期:2015-11-30 修回日期:2016-04-27 接受日期:2016-01-25 出版日期:2016-05-17 发布日期:2016-05-17
  • 通讯作者: 杨玲
  • 基金资助:
    山东省农业良种工程项目“东平湖鲤种质资源发掘与良种选育”;山东省现代农业产业技术体系创新团队“鱼类产业创新团队建设”(SDAIT-14-011-03)。

Karyotype of Cyprinus carpio in Dongping Lake

Lu Hong, Meng Qinglei, Yang Ling   

  1. (Shandong Provincial Key Laboratory of Freshwater Fishery Genetic Breeding, Freshwater Fishery Research Institute of Shandong Province, Jinan 250013)
  • Received:2015-11-30 Revised:2016-04-27 Accepted:2016-01-25 Online:2016-05-17 Published:2016-05-17

摘要: 为了解东平湖鲤(Cyprinus carpio)的遗传组成,为发掘、保护及合理利用其种质资源提供细胞遗传学方面的基础资料,采用腹腔注射植物血球凝集素(PHA)和秋水仙素的肾细胞体内培养法,以东平湖鲤头肾组织为材料,通过空气干燥法制备染色体标本,进行染色体组型分析。东平湖鲤的染色体数2n=100,核型公式为2n=20m+38sm+20st+22t,染色体总臂数(NF)为158。通过比较分析认为,东平湖鲤在鲤属鱼类中属于较为进化的类群。

关键词: 苹果, 苹果, 叶片, 发育, 矿质营养, 相关性

Abstract: To understand the genetic composition of carp (Cyprinus carpio) in Dongping Lake and provide basic data in cytogenetics for exploration, protection and appropriate utilization of its germplasm resources, karyotype of carp (Cyprinus carpio) in Dongping Lake was studied. PHA and colchicines were injected into head-kidneys and then the chromosome samples were prepared by air-drying technique. The results showed that the chromosome number of Cyprinus carpio in Dongping Lake was 2n=100; the karyotype formula was 2n=20m 38sm 20st 22t. The total number of chromosome arms (NF) was 158. Compared with the karyotype of other fishes in Cyprinus, it was concluded that Cyprinus carpio in Dongping Lake was a more evolutionary group among fishes in Cyprinus.

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