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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (23): 10-15.doi: 10.11924/j.issn.1000-6850.casb16050091

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

柱前衍生-高效液相色谱法测定饲料中牛磺酸的研究

殷秋妙,何绮霞,吴维煇,王威利,续 倩,张 展   

  1. 广东省农业科学院农产品公共监测中心,广东省农业科学院农产品公共监测中心,广东省农业科学院农产品公共监测中心,广东省农业科学院农产品公共监测中心,广东省农业科学院农产品公共监测中心,广东省农业科学院农产品公共监测中心
  • 收稿日期:2016-05-16 修回日期:2016-08-01 接受日期:2016-07-11 出版日期:2016-08-10 发布日期:2016-08-10
  • 通讯作者: 殷秋妙
  • 基金资助:
    广东省农业领域科技计划项目“蛋白质饲料原料质量安全监测技术的集成与示范”(2013B020306001)；广东省主体科研机构创新能力建设专项资金(2060299)。

Determination of Taurine Content in Feeds by HPLC After Derivatization with Dansyl Chloride

  • Received:2016-05-16 Revised:2016-08-01 Accepted:2016-07-11 Online:2016-08-10 Published:2016-08-10

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461

摘要： 为探讨GB 5413.26—2010 中丹磺酰氯柱前衍生HPLC法测定饲料中牛磺酸的可行性，以鸡配合饲料、猪配合饲料、水产配合饲料、浓缩饲料和预混合饲料五类代表性饲料样品为研究对象，通过梯度添加回收试验和基质效应影响的研究，确定了各类饲料的适宜称样量为猪鸡配合饲料2~4 g，其他饲料1 g。对方法的各项性能指标进行了考察，结果表明：在牛磺酸1~100 mg/L 范围，工作曲线线性良好，相关系数0.9990。该方法检测限为30 mg/kg，定量限100 mg/kg，添加回收率为78%~110%，精密度为1.98%~5.35%。该方法操作简单、灵敏、仪器普适通用，适宜于各种饲料基质中牛磺酸的测定。

关键词: 大豆异黄酮, 大豆异黄酮, ‘荷斯坦奶牛’, 泌乳性能, 免疫功能, 抗氧化

Abstract: This study aimed to test whether the taurine determination method in GB 5413.26—2010 could be applied in determination of taurine in feeds. After derivatization with dansyl chloride, high performance liquid chromatography (HPLC) was conducted for taurine determination in chicken, swine and aquatic formula feed, concentrated feed and premixed feed. The reclamation test and matrix effect analysis showed that the proper sampling amount was 2-4 g for chicken and swine formula feeds, and 1 g for other feeds. Then the performance index of this method was tested. The results showed that when the concentration of taurine was 1-100 mg/L, the working curve had a good linear relation with the correlation coefficient being 0.9990. The limit of detection was 30 mg/kg, and the limit of quantitation was 100 mg/kg. The average recovery rate and relative standard deviation were 78%-110% and 1.98%-5.35%, respectively. The established method was sensitive, simple for operation, wide for application and convenient for fast taurine determination in different feeds.

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