解淀粉芽孢杆菌GB58对水稻纹枯病的防效及菌剂载体筛选

doi:10.11924/j.issn.1000-6850.casb16060029

中国农学通报 ›› 2017, Vol. 33 ›› Issue (11): 119-125.doi: 10.11924/j.issn.1000-6850.casb16060029

所属专题：水稻

解淀粉芽孢杆菌GB58对水稻纹枯病的防效及菌剂载体筛选

卢钰升,顾文杰,蒋瑞萍,徐培智,解开治,孙丽丽

(广东省农业科学院农业资源与环境研究所/农业部南方植物营养与肥料重点实验室/广东省养分资源循环利用与耕地保育重点实验室,广州 510642）

收稿日期:2016-06-07 修回日期:2017-03-21 接受日期:2016-08-25 出版日期:2017-04-26 发布日期:2017-04-26
通讯作者: 顾文杰
基金资助:
云浮市科技计划项目“功能性（抗病、促生）生物有机肥的研发与应用”(201402-2)；广东省科技计划项目“河源龙川县山区冷浸田土壤改良与水稻产量提升技术研究及示范推广”(2015A020224010)；广东省科技计划项目“基于菜园退化土壤的功能性微生物接种剂研发与应用”(2013B020310001)；广东省科技计划项目“多元生物有机肥料制备关键技术研究及产业化示范”(2015B020237007)；广东省科技计划项目“广东省新型肥料与高效施肥工程技术研究中心建设”(2014B090904068)；广东省科技计划项目“广东省新型肥料科技创新与服务平台建设”；广东省农科院院长基金项目(201715)。

Control Effect of Bacillus amyloliquefaciens GB58 on Rice Sheath Blight andScreening of Microbial Agent Carrier

Lu Yusheng, Gu Wenjie, Jiang Ruiping, Xu Peizhi, Xie Kaizhi, Sun Lili

(Institute of Agricultural Resources and Environment/Guangdong Academy of Agricultural Sciences Key Laboratory of Plant Nutrition and Fertilizer in South Region, Ministry of Agriculture/Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation, Guangzhou 510640)

Received:2016-06-07 Revised:2017-03-21 Accepted:2016-08-25 Online:2017-04-26 Published:2017-04-26

摘要/Abstract

摘要： 旨在对解淀粉芽孢杆菌GB58在水稻纹枯病上的盆栽防治效果和最佳菌剂载体进行研究，明确其应用前景。采用盆栽试验测定GB58对水稻纹枯病的病斑高率和在水稻上的定殖能力，测定GB58在菌剂固定载体的吸附能力和吸附稳定性。盆栽试验结果表明，GB58在水稻病株上有良好的定殖效果，定殖数最大达到2.22×10⁷ cfu/g。与喷施井冈霉素处理相比，GB58对水稻纹枯病的防效在病害发生后20、40天分别显著提高了20%、24%。GB58在固体菌剂吸附载体的研究表明：结合菌体吸附量、存活率和抑菌活性进行分析，有机载体吸附效果好于无机载体；同时综合考虑应用价值，选择玉米粉、有机肥为菌剂吸附载体较好。GB58对水稻纹枯病具有较好的生防效果。采用玉米粉和有机肥作为固体菌剂吸附载体，具有经济、高效的特点，有较大的应用价值与开发潜力。

关键词: 鹰嘴豆, 鹰嘴豆, 文献计量分析, 学科态势, 学科情报, 学科服务

Abstract: [Objective] This study aims at researching on the effect of the biocontrol outcome against rice sheath blight disease of the Bacillus amyloliquefaciens GB58 and its most suitable microbial agent carrier to determine the application prospect.[Method] The pot experiment method was adopted to determine the relative lesion height (RLH) of rice sheath blight, colonization ability of rice. The adsorption capacity and stability of GB58 on microbial agent carrier was determined. [Result]The pot experiment result showed that GB58 had an optimistic colonization effect at 2.2×107 cfu/g of the maximum on the diseased plants. Compared to validamycin treatment, the biocontrol effect of GB58 was raised significantly by 20% and 24% respectively in 20d and 40d after inoculating the pathogen. The test on solid microbial agent carrier of GB58 suggested that, taking cell absorption amount, survival rate and antimicrobial activity into consideration, the absorption effect of the organic carrier was better than that of the inorganic. While economic factors were considered comprehensively, the choice of corn meal and organic fertilizer would be ideal.S[Conclusion] Our results came to the conclusion that GB58 had a biocontrol advantage over rice sheath blight disease, while corn meal and organic fertilizer, effective and economical as the microbial agent carrier, had wide application value and great potential.

中图分类号:

S182

卢钰升,顾文杰,蒋瑞萍,徐培智,解开治,孙丽丽. 解淀粉芽孢杆菌GB58对水稻纹枯病的防效及菌剂载体筛选[J]. 中国农学通报, 2017, 33(11): 119-125.

Lu Yusheng,Gu Wenjie,Jiang Ruiping,Xu Peizhi,Xie Kaizhi and Sun Lili. Control Effect of Bacillus amyloliquefaciens GB58 on Rice Sheath Blight andScreening of Microbial Agent Carrier[J]. Chinese Agricultural Science Bulletin, 2017, 33(11): 119-125.

参考文献

[1] 刘薇,杨超,邹剑锋,等.水稻纹枯病生物防治研究进展[J].广西农业科学,2009(5):512-516.
[2] Miyake I. Studies on the fungus diseases of rice in Japan[J]. Journal of the College of Agriculture,1910(2):237-276.
[3] Lee F N, Rush M C. Rice sheath blight: A major rice disease.[J]. Plant Disease,1983,67(7):829-833.
[4] Rush M C, Lindberg G D. Rice disease research[J]. Rice J, 1996,77:49-52.
[5] Groth D E. Effects of cultivar resistance and single fungicide application on rice sheath blight, yield, and quality[J]. Crop 发 protection,2008,27(7):1125-1130.
[6] Bernardes-De-Assis J, Storari M, Zala M, et al. Genetic structure of populations of the rice-infecting pathogen Rhizoctonia solani AG-1 IA from China[J]. Phytopathology,2009, 99(9):1090-1099.
[7] Mew T W, Cottyn B, Pamplona R, et al. Applying rice seed-associated antagonistic bacteria to manage rice sheath blight in developing countries[J]. Plant disease,2004,88(5):557-564.
[8] 陈志谊,刘荣,刘永锋.水稻纹枯病拮抗细菌B-916的选育[J].中国生物防治,2003(1):15-18.
[9] Vijay Krishna Kumar K, Yellareddygari S, Reddy M S, et al. Efficacy of Bacillus subtilis MBI 600 Against Sheath Blight Caused by Rhizoctonia solani and on Growth and Yield of Rice[J]. Rice Science,2012,19(1):55-63.
[10] 陈宏州,杨敬辉,朱桂梅,等.水稻纹枯病菌拮抗放线菌的筛选与生防潜能评价[J].江苏农业科学,2009(5):141-143.
[11] Kazempour M N. Biological Control of Rhizoctonia solarti, the Causal Agent of Rice Sheath Blight by Antagonistic Bacteria in Greenhouse and Field Conditions[J]. Plant Pathology Journal,2004,3(2):88-96.
[12] Luo J Y, Xie G L, Li B, et al. Gram-positive bacteria associated with rice in China and their antagonists against the pathogens of sheath blight and bakanae disease in rice[J]. Rice Sci., 2005,12(3):213-218.
[13] Wang H, Wen K, Zhao X, et al. The inhibitory activity of endophytic Bacillus sp. strain CHM1 against plant pathogenic fungi and its plant growth-promoting effect[J]. Crop protection,2009,28(8):634-639.
[14] Kanjanamaneesathian M, Wiwattanapatapee R, Pengnoo A, et al. Efficacy of Novel Formulations of Bacillus megaterium in Suppressing Sheath Blight of Rice Caused by Rhizoctonia solani.[J]. Plant Pathology Journal,2007,6(2).
[15] Al-Taweil H I, Osman M B, Hamid A A, et al. Development of microbial inoculants and the impact of soil application on rice seedlings growth[J]. American Journal of Agricultural and Biological Sciences,2009, 4(1):79.
[16] 陈志谊,陆凡,刘永锋,等.防治水稻病害微生物农药纹曲宁的研制及产业化[J].江苏农业学报,2003(2):108.
[17] 张学君,凌宏通,李洪连,等.生物农药麦丰宁B_3对小麦纹枯病菌的抑制作用[J].植物病理学报,1994(4):361-366.
[18] 邓晓皋.吸附法固定微生物细胞的研究进展[J].生物工程进展,1993(4):7-10.
[19] 翁媛媛,陈洪章.惰性吸附载体固态发酵细菌纤维素的研究[J].纤维素科学与技术,2010(4):1-7.
[20] 杨旭升.不同吸附载体对大豆根瘤菌剂质量影响研究[J].内蒙古科技与经济,2012(13):121.
[21] Manohar S, Kim C, Karegoudar T. Enhanced degradation of naphthalene by immobilization of Pseudomonas sp. strain NGK1 in polyurethane foam[J]. Applied microbiology and biotechnology,2001,55(3):311-316.
[22] 王新,李培军,宋守志,等.固定化微生物技术在环境工程中的应用研究进展[J].环境污染与防治,2005(7):66-68.
[23] 卢钰升,顾文杰,蒋瑞萍,等.一株生防细菌GB58的鉴定与抑菌能力测定[J].中国农学通报,2016(6):198-204.
[24] Park D, Sayler R J, Hong Y, et al. A method for inoculation and evaluation of rice sheath blight disease[J]. Plant Disease,2008, 92(1):25-29.
[25] 胡杨,张余杰,唐建林.一株水稻纹枯病拮抗细菌A168定殖作用研究[J].吉林农业,2012(3):44-45.
[26] Willocquet L. Effect of bacterial antagonists on infection of rice by sheath blight[C]. International Rice Research Institute, 1995.
[27] 李湘民,许志刚,Mew T. W. 稻株上拮抗细菌的定殖及其对土著细菌的影响[J].生态学报,2008(8):3868-3874.
[28] 刘邮洲,陈志谊,刘永锋,等.拮抗细菌B-916对水稻病原菌的抑制效果及其定殖动态研究[J].江苏农业科学,2005(6):48-49.
[29] 李湘民,胡白石,许志刚,等.拮抗细菌Bacillus subtilis B5423-R抑制水稻纹枯病的阈值群体数量[J].中国水稻科学,2003(4):71-75.
[30] 张德涛,彭正凯,曹琦琦,等.3株拮抗菌在水稻植株上的定殖能力及对纹枯病的防效[J].西北农林科技大学学报:自然科学版,2012(2):97-102.
[31] Weller D M. Biological control of soilborne plant pathogens in the rhizosphere with bacteria[J]. Annual review of phytopathology,1988,26(1):379-407.
[32] 王志刚,爱德华,张颖,等.阿特拉津土壤污染修复菌剂载体材料的筛选与应用[J].环境工程学报,2014(8):3487-3494.
[33] 马梅荣,陈雷,宣世伟,等.XM固态菌剂载体选择的初步研究[J].哈尔滨工业大学学报,2005(2):259-261.
[34] 张昊,王洪涛,张利兰,等. Phanerochaete chrysosporium吸附载体的选择及染料降解研究[J].环境工程学报,2010(12):2755-2760.
[35] 刘方春,邢尚军,马海林,等.不同有机废弃物作为假单胞菌YT3吸附载体的研究[J].农业环境科学学报,2013(6):1194-1200.
[36] 乔焕英,丁传雨,沈其荣,等.拮抗菌发酵的生物有机肥对茄子生长和青枯病的防治效果[J].土壤,2012(3):461-466.

解淀粉芽孢杆菌GB58对水稻纹枯病的防效及菌剂载体筛选

Control Effect of Bacillus amyloliquefaciens GB58 on Rice Sheath Blight andScreening of Microbial Agent Carrier

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	张欣蕊, 冯启鑫, 安绮沄, 程丽, 李冲伟. 基于Web of Science紫苏研究进展与态势分析[J]. 中国农学通报, 2022, 38(4): 144-152.
[2]	马笑, 张世浩, 张芬, 刘发波, 梁涛, 王孝忠, 陈新平. 基于Web of Science对蔬菜系统活性氮损失研究的文献计量分析[J]. 中国农学通报, 2022, 38(26): 124-132.
[3]	翁晓虹, 隋心. 基于Web of Science的森林土壤微生物多样性研究趋势分析[J]. 中国农学通报, 2022, 38(10): 157-164.
[4]	李静超, 佘容, 杨晓燕. 土壤微生物宏基因组研究现状——基于Citespace文献计量分析[J]. 中国农学通报, 2021, 37(28): 142-152.
[5]	贾宽宽, 舒英格, 张忠梁, 王渊, 任明慧. 基于CNKI数据库的茶园土壤酸化文献计量分析[J]. 中国农学通报, 2021, 37(20): 119-125.
[6]	吴汉卿, 张宝贵, 王学霞, 曹兵, 陈立娟, 刘杰, 陈延华. 文献计量分析在快速检索文献中的应用——以土壤氨挥发为例[J]. 中国农学通报, 2021, 37(1): 147-157.
[7]	吴名杰, 王贺, 伍一宁, 王伟华, 邹红菲. 基于文献计量的土壤环境中多环芳烃降解的研究现状[J]. 中国农学通报, 2020, 36(5): 60-67.
[8]	刘秋霞,吴汉卿,黄正来. 基于全球文献计量的小麦响应气候变暖的研究[J]. 中国农学通报, 2019, 35(23): 142-151.
[9]	伍一宁,钟海秀,王贺,许楠,李金博,付晓宇,邹红菲. 基于文献计量的土壤动物发展研究现状与趋势₁₎[J]. 中国农学通报, 2018, 34(9): 74-80.
[10]	周伏忠,冯菲,宁萌,王雪妍,刁文涛,陈晓飞. 鹰嘴豆浆液蛋白发酵与酶解效果的对比分析[J]. 中国农学通报, 2015, 31(29): 54-58.
[11]	杨金凤,吕俊生,张小云. 鹰嘴豆研究的文献计量分析[J]. 中国农学通报, 2015, 31(18): 96-110.
[12]	李翠莲. 鹰嘴豆抗氧化多肽与其它抗氧化剂的协同作用[J]. 中国农学通报, 2008, 24(10): 57-60.