小麦K-CMS保持系的SRAP鉴定及遗传多样性分析

doi:10.11924/j.issn.1000-6850.casb16070044

中国农学通报 ›› 2017, Vol. 33 ›› Issue (20): 5-10.doi: 10.11924/j.issn.1000-6850.casb16070044

所属专题：小麦

小麦K-CMS保持系的SRAP鉴定及遗传多样性分析

范可章,邢斌,赵翔,马同富,卢良峰,蔡健

阜阳师范学院生命科学学院,阜阳师范学院生命科学学院,阜阳师范学院生命科学学院,阜阳师范学院生命科学学院,河南农业职业学院现代农业工程系,阜阳师范学院生命科学学院

收稿日期:2016-07-09 修回日期:2016-09-20 接受日期:2016-09-21 出版日期:2017-07-18 发布日期:2017-07-18
通讯作者: 蔡健
基金资助:
国家星火计划项目“皖北草莓良种引种栽培及工厂化育苗示范推广”(2013GA710018)；安徽省高校省级自然科学基金重点项目“小麦TCMS 相关基因orf256/cox1 的克隆及功能分析”(KJ2014A194)；安徽省高校省级自然科学基金项目“皖北农作物秸秆栽培食用菌综合利用研究” (KJ2013B197)。

Analysis of Genetic Diversity and Identification of Maintainer Line for K-CMS Germplasm Resources in Wheat Using SRAP Markers

Received:2016-07-09 Revised:2016-09-20 Accepted:2016-09-21 Online:2017-07-18 Published:2017-07-18

摘要/Abstract

摘要： 为更好地对小麦K-CMS保持系的遗传背景进行分析，筛选优良亲本，以进一步提高小麦杂交育种的效率。利用SRAP技术，对30 份小麦K-CMS保持系的遗传背景进行研究，以SRAP标记在30 个品种之间扩增的多态性位点数据，采用Nei 和Li 的方法，计算品种间的遗传距离；以2 年田间试验得到的品种株高、穗长、小穗数、穗下节长、穗粒草重、穗粒数、穗粒重、旗叶长等表现型性状平均数经正态标准化后，采用欧氏距离计算品种间遗传距离，再比较分析。分析表明，82 对SRAP引物揭示出多态性，多态率68.3%，获得208 条多态性谱带，平均每对引物产生3.68 条多态性谱带，表现出较高的多态性。基于分子标记的聚类分析结果与系谱分析结果基本一致。若以整个遗传距离的总平均数作尺度对聚类图的结果进行分类，大致可分为6 类。Mantel 检测表明，SRAP标记数据计算的遗传距离矩阵和表现型计算的遗传距离矩阵存在极显著的相关性0.8123(t=11.325>t0.01)。SRAP标记是检测品种间遗传差异的有效方法，可为小麦K-CMS保持系种质资源遗传差异的研究提供理论依据。研究还证实，一个骨干亲本与由其衍生出来的品种（系）之间的遗传差异一般较小。

关键词: 雾, 雾, 霾, 气候特征, 统计分析方法

Abstract: To analyze the genetic background of the maintainer line for K-type cytoplasmic male sterility (CMS) in wheat, screen the excellent parents for breeding, and improve hybrid breeding efficiency in wheat, the genetic diversity of 30 maintainer lines for K-CMS germplasm resources of wheat were assessed using SRAP markers in this study. SRAP markers were used to amplify the polymorphic loci in the 30 lines, the genetic distances between cultivars were calculated by using Nei and Li methods. In 2 years field experiment, the average of phenotypic traits about plant height, panicle length, spikelet number, spike length, spike grass weight, grain number and grain weight per spike, length of flag leaf and so on were carried out in normal standard, using Euclidean distance to calculate the genetic distance among varieties, and comparative analysis was conducted. Analysis showed that 82 pairs of SRAP primers revealed polymorphism, the polymorphism rate was 68.3% , 208 polymorphic bands were obtained, the average of each pair of primers produced 3.68 polymorphic bands, showed high polymorphism. The results of cluster analysis based on molecular markers and pedigree analysis results were consistent. Taking the total average genetic distance as the scale, the results of cluster graph could be divided into 6 categories. Mantel detection showed that there was a very significant correlation between the genetic distance matrix of SRAP marker data and the expression pattern of the genetic distance matrix (r =0.8123,t =11.325>t 0.01). SRAP marker is an effective method to detect the genetic difference among cultivars, which can provide theoretical basis for the research of genetic difference of germplasm resources of K-CMS in wheat. Studies also confirm that the genetic difference between a backbone parent and the breed derived from it is generally small.

范可章,邢斌,赵翔,马同富,卢良峰,蔡健. 小麦K-CMS保持系的SRAP鉴定及遗传多样性分析[J]. 中国农学通报, 2017, 33(20): 5-10.

参考文献

[1] 黄群英. 中国两系法籼稻杂交水稻育种的研究新进展[J]. 科技导报, 1995, ( 11): 46- 48.
[2] Gomma A S, Khalil O I S. Genetics and another score and seed s et and their correlation in certain wheat hybrides with T. timopheevi cytoplasm [J] . Ag r Res Revi, 1980, 58: 1- 20.
[3] Song X Y(宋喜悦), Fang P(方鹏), Ma L J(马翎健), et al. A comparative study about K type wheat male sterile lines of Non 1B/1R type and type 1B/1R[J]. Journal of northwest agriculture and forestry university of science and technology(Natural science edition)西北农林科技大学学报(自然科学版),2002,30(1):1-4.
[4] He B R(何蓓如)．A selection method of wheat male sterile maintainer lines and sterile lines [P]. Chinese Patent(中国专利),ZL94116837.X,1994.
[5] Lu L F(卢良峰),Zhu F R(朱凤荣). Studies on selection of restorer and maintainer for K-type cytoplasmic male sterility wheat [J]. Henan agricultural science(河南农业科学),2004年10期：3-5(in Chinese with English abstract).
[6] He B R(何蓓茹),Ning Y H(宁毓华),Liu S D(刘曙东). The primary research on 1B/1R type wheat cytoplasmic male sterility [J]. Journal of Xibei Agricultural University(西北农业大学学报),1987,15(3)：107-109(in Chinese with English abstract).
[7] Cao S L(曹士亮),Cao J S(曹靖生),Wang C B(王成波),et al. Review on operation proceduresof maize SSR molecular marker technology [J]. Chinese Agricultural Science Bulletin(中国农学通报),2012,28(15)：1-4(in Chinese with English abstract).
[8] Li G,Quiros C F．Sequence-related amplified polymorphism(SRAP) ,A new markersystem based on a simple PCR reaction: itsapplication to mapping and gene tagging inBrassica[J]．Theoreticaland Applied Genetics,2001,103: 455－461
[9] Lin Z X,Zhang X L,Nie Y C,et al．Construction ofa genetic linkage map for cotton based on SRAP[J]. ChineseScience Bulletin,2003,48 ( 19) : 2064-2068
[10] Li G,Gao M,Yang B,et al．Gene for gene alignmentbetween the Brassica and Arabidopsisgenomes by direct transcription mapping[J]．Theoretical and Applied Genetics,2003,107: 271－282
[11] Ferriol M, Picó B, Nuez F． Genetic diversity of a germplasmcollection of Cucurbitapepo using SRAP and AFLP markers[J]．Theoretical and Applied Genetics,2003,107: 256-261
[12] Ge S,Hong D Y. Genetic diversity and its detection, //BiodiversityCommittee of ChineseAcademy of Sciences．Principles andMethodologies of Biodiversity Studies[M]．Beijing: Chinese Scienceand Technology Press,1994,123-140
[13] Wang L H(王林海),Wang X W(王晓伟),Zhan K H(詹克慧). Cluster analysis of some wheatgermplasms in Huang-Huai area based on agronomic traits [J]. Chinese Agricultural Science Bulletin(中国农学通报),2008,24(4)：186-190(in Chinese with English abstract).
[14] Ma D F(马代夫),Li Q(李强),Li X Y(李秀英),et al．Selection of parents for breeding of sweetpotato food varieties of high carotene content[J]．Scientia Agricultural Sinica( 中国农业科学),2009,42(3): 798－808(in Chinese with English abstract).
[15]Li G,Quiros C F．Sequence-related amplified polymorphism(SRAP) , A new marker system based on a simple PCR reaction: itsapplication to mapping and gene tagging in Brassica[J]．Theoreticaland Applied Genetics, 2001, 103:455-461
[16]Li Q(李强),Liu Q C(刘庆昌),Zhai H(翟红),et al．Genetic diversity in main parents of sweetpotato in china as revealed by ISSR marker[J]．ACTA AGRONOMICA SINICA(作物学报),2008,34(6):972－977(in Chinese with English abstract).
[17]Nei M,Li W H． Mathematical model for studying genetic variationin terms of restrictionendonucleases[J]． Proceedings of the NationalAcademy of Sciences of the United Statesof America,1979,76:569－572
[18]Zhong Yang(钟扬),Chen Jiakuan(陈家宽),Huang Deshi(黄德世)．The classification numberof methods and procedures[M]．Wuchang: Wuhan Uiniversity Press,1990:47(in chinese).
[19]Tang Qiyi(唐启义),Feng Mingguang(冯明光)． Practical statistical analysis and DPS dataprocessing system[M]．Beijing: Science press,2002: 551－603(in chinese).2002: 551－603
[20] Huang S T(黄寿松),Li W L(李万隆),Xu H(徐浩),et al. The development of a blue marked nucleus male sterile line and its maintainer in bread wheat [J]. ACTA AGRONOMICA SINICA(作物学报),1991,17(2)：81-87(in Chinese with English abstract) .
[21]Su C H(苏春华),Li Y M(李育明),Huang Y D(黄迎冬),et al．The principal componentanalysis and cluster analysis of sweetpotato varieties as parents[J]．Rain Fed Crops(杂粮作物),2007,27 (6) : 405 －409(in Chinese with English abstract).
[22]Wu J(吴洁),Tan W F(谭文芳),Yan W Z(阎文昭),et al． Geneti relationship of sweetpotatocultivars analysised by sequence-related amplified polymorphism[J]．Journal of Sichuan University(Natural Science Edition)(四川大学学报: 自然科学版),2007,44(4) : 878－882(in Chinese with English abstract).

小麦K-CMS保持系的SRAP鉴定及遗传多样性分析

Analysis of Genetic Diversity and Identification of Maintainer Line for K-CMS Germplasm Resources in Wheat Using SRAP Markers

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