隐甲藻生产二十二碳六烯酸的研究进展

doi:10.11924/j.issn.1000-6850.casb18050042

摘要/Abstract

摘要： 二十二碳六烯酸(docosahexaenoicacid，DHA)是具有重要生理功能而人体自身无法合成的一类多不饱和脂肪酸，而隐甲藻(Crypthecodinium cohnii)是生产二十二碳六烯酸的理想原料之一。本文为了将来开展隐甲藻规模化生产DHA研究，综合分析了隐甲藻种质、DHA生物合成途径、培养条件对DHA积累的影响等方面的问题。具体为（1）组成隐甲藻的同胞体DHA产量不同，可利用诱变的方式筛选突变体获得新的优良品系；（2）隐甲藻DHA的生物合成与其他脂肪酸的生物合成不同，对其合成途径进行了推测，可能为PKS途径；（3）环境条件，包括温度、光照、pH值、溶氧量、接种量、消泡处理等，以及培养基组成，包括碳源、氮源、无机盐及微量元素等培养条件均会影响藻体中DHA的含量。诱变是获得高产DHA藻株的可行性方法,以此提出了对隐甲藻生产DHA的研究展望。

关键词: 生菜, 生菜, 采后生理, 保鲜, 贮藏, 运输

Abstract: Docosahexaenoicacid (DHA) is a class of polyunsaturated fatty acid with important physiological functions which the body could not synthesis. Exogenous supplementation of DHA can enhance immune function, physical fitness of human beings. Crypthecodinium cohnii is one of the ideal raw materials for the production ofDHA. In order to carry out DHA research on the large-scale production of Crypthecodinium cohnii in the future, this paper comprehensively analyzed the problems of the species of Cryptococcus, DHA biosynthesis, and the influence of culture conditions on DHA accumulation.Specifically, (1) the DHA production of the siblings of the cryptophyta is different, and the mutants can be screened to obtain new excellent lines by mutagenesis; (2) the biosynthesis of DHA is different from the biosynthesis of other fatty acids, The synthetic route is speculated, possibly the PKS pathway; (3) environmental conditions, including temperature, light, pH, dissolved oxygen, inoculum, defoaming, etc., and medium composition, including carbon source, nitrogen source, Culture conditions such as inorganic salts and trace elements affect the content of DHA in algae. Mutagenesis is a feasible method for obtaining high-yield DHA strains, and put forward the research prospect of DHA production by Cryptophyta.

贾文斌,辛富刚,孙玉霞,姚川,丁汉凤. 隐甲藻生产二十二碳六烯酸的研究进展[J]. 中国农学通报, 2019, 35(30): 84-90.

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