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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (18): 102-108.doi: 10.11924/j.issn.1000-6850.casb2020-0054

所属专题： 生物技术

• 生物科学 • 上一篇    下一篇

栽培三七内生真菌优势种群分离鉴定

李维蛟1(), 任可2, 浦仕彪1()   

  1. 1云南中医药大学,昆明 650500
    2西南大学农学与生物科技学院,重庆 400715
  • 收稿日期:2021-01-18 修回日期:2021-03-16 出版日期:2021-06-25 发布日期:2021-07-13
  • 通讯作者: 浦仕彪
  • 作者简介:李维蛟,男,1978年出生,山西孝义人,博士,研究方向：药用植物病理学及道地濒危中草药开发。通信地址：650500 云南省昆明市呈贡新区雨花路1076号 云南中医药大学,E-mail： liweijiao163@163.com。
  • 基金资助:
    云南省高层次人才培养支持计划“云南省万人计划青年拔尖人才专项”(YNWR-QNBJ-2018-190);云南省科学技术厅科技人才与平台计划省级工程技术研究中心建设项目“云南省道地濒危中药材繁育与栽培工程技术研究中心”(2016DH011);云南省中医药学分子生物学重点实验室(筹)开放课题基金“云南重楼内生真菌具宿主活性成分探索研究”(2019DG016)

Endophytic Fungi from Panax notoginseng (Burk.) F.H.Chen: Isolation and Identification

Li Weijiao1(), Ren Ke2, Pu Shibiao1()   

  1. 1Yunnan University of Chinese Medicine, Kunming 650500
    2College of Agronomy and Biotechnology, Southwest University, Chongqing 400715
  • Received:2021-01-18 Revised:2021-03-16 Online:2021-06-25 Published:2021-07-13
  • Contact: Pu Shibiao

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摘要：

栽培三七为五加科人参属多年生草本药用植物,旨在从药用植物栽培三七中分离出能够产生与宿主植株相同或者相似的生物活性物质的内生真菌菌株。以栽培三七为材料,开展栽培三七内生真菌的分离、纯化,依据传统形态特征并结合真菌的ITS序列进行菌株鉴定。初步从栽培三七中共分离得到内生真菌24株,鉴定为12属（种）,栽培三七不同器官组织的内生真菌种类和数量差异较大,根和叶中最多,茎中较少。其中从叶片中分离出11株,6属;茎中分离出1株,1属;根中12株,5属。统计结果表明叶片中分离出的菌株优势种为普通青霉菌(Penicillium commune)、胶孢刺盘孢菌(Colletotrichum gloeosporioides),均占叶片菌株分离的27.28%;须根中分离出的菌株优势种为球毛壳菌(Chaetomidium arxii),占须根菌株分离的72.73%。栽培三七内生真菌具有丰富的生物多样性,实验结果为进一步探讨栽培三七内生真菌与生理活性物质之间的关系提供材料基础和实验参数。

关键词: 三七, 内生真菌, 分离鉴定, ITS, 药用植物

Abstract:

Panax notoginseng (Burk.) F.H. Chen is a perennial herbaceous medicinal plant of ginseng in Acanthopanax family. The study aims to isolate endophytic fungus strains which can produce the same or similar biological active substances as the host plant from the medicinal plant. P. notoginseng was used as material. The endophytic fungi strains of P. notoginseng was isolated and purified, and then the endophytic fungi strains were identified based on the traditional morphological characteristics and ITS sequence. A total of 24 strains of endophytic fungi were isolated from cultivated P. notoginseng, and they were identified as 12 genera (species). The types and numbers of endophytic fungi in different organs and tissues of cultivated P. notoginseng were quite different, the roots and leaves had the most, and the stems had less. Among them, 11 species and 6 genera were isolated from leaves, 1 species and 1 genus from stems, 12 species and 5 genera from roots. The statistical results showed that Penicillium commune and Colletotrichum gloeosporioides were the dominant species isolated from leaves, accounting for 27.28% of the isolates from leaves; the dominant strains isolated from the fibrous roots were Chaetomidium arxii, accounting for 72.73% of the isolated root strains. The cultivated P. notoginseng endophytic fungi have rich biodiversity, thus the experimental results could provide a material basis and experimental parameters for further exploring the relationship between cultivated P. notoginseng endophytic fungi and physiological active substances.

Key words: Panax notoginseng (Burk.) F.H. Chen, endophytic fungi, isolation and identification, ITS, medicinal plant

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