立枯丝核菌诱导下深色有隔内生真菌A024代谢组学分析

doi:10.11924/j.issn.1000-6850.casb2020-0190

中国农学通报 ›› 2021, Vol. 37 ›› Issue (8): 25-32.doi: 10.11924/j.issn.1000-6850.casb2020-0190

所属专题：生物技术

立枯丝核菌诱导下深色有隔内生真菌A024代谢组学分析

宋小双¹^,²(), 遇文婧¹^,², 闵凯³, 周琦¹^,², 邓勋¹^,²(), 刘艳红¹^,², 姜瑞凤¹^,²

¹黑龙江省森林保护研究所,哈尔滨 150040
²黑龙江省森林草原火灾及病虫害防控重点实验室,哈尔滨 150040
³大庆油田矿区服务事业部,黑龙江大庆 163000

收稿日期:2020-06-18 修回日期:2020-08-27 出版日期:2021-03-15 发布日期:2021-03-16
通讯作者: 邓勋
作者简介:宋小双,女,1977年生出生,河北迁安人,副研究员,博士,主要从事森林病理学和土壤微生物研究。通信地址：150040 黑龙江省哈尔滨市哈平路134号黑龙江省林科院森林有害生物防治研究中心,Tel：0451-86630340,E-mail： sxshappy@126.com。
基金资助:
国家自然科学基金项目“深色有隔内生真菌提高樟子松抗病性的生理及分子机制”(31670649);国家自然科学基金项目“褐环乳牛肝菌与菌根辅助细菌互作对樟子松促生抗病作用机制”(31700564);黑龙江省林科院人才基金项目“外生菌根菌褐环乳牛肝菌(Suillus luteus)快速分子检测技术”(2015R01)

Metabonomics Analysis of Dark Septate Endophytes Phialocephala bamuru A024 Under the Induction of Rhizoctonia solani Kühn

Song Xiaoshuang¹^,²(), Yu Wenjing¹^,², Min Kai³, Zhou Qi¹^,², Deng Xun¹^,²(), Liu Yanhong¹^,², Jiang Ruifeng¹^,²

¹Heilongjiang Institute of Forest Protection, Harbin 150040
²Heilongjiang Key Laboratory of Forest Grassland Fire and Pest Control, Harbin 150040
³Daqing Oilfield Logistic Administration, Daqing Heilongjiang 163000

Received:2020-06-18 Revised:2020-08-27 Online:2021-03-15 Published:2021-03-16
Contact: Deng Xun

摘要/Abstract

摘要：

为研究深色有隔内生真菌(Phialocephala bamuru)A024对立枯丝核菌(Rhizoctonia solani Kühn)的抑制机制,本研究从采用LC-MS分析平台对立枯丝核菌诱导条件下深色有隔内生真菌A024的非靶向代谢组进行分析。结果表明菌株A024代谢产物包括9大类,27小类,筛选得到52个差异代谢物,同对照相比,诱导后上调的31个,下调的21个,其中黄酮类化合物、L-苹果酸、磷酸鸟苷、烟酸等显著上调。差异代谢产物的KEGG富集分析中花生四烯酸代谢、组氨酸和嘌呤类生物碱的生物合成、植物次生代谢产物的生物合成、萜类和聚酮类生物碱的生物合成等显著富集。筛选得到多个差异显著性代谢产物及其富集的代谢通路,为进一步研究其对立枯丝核菌的抑制作用机制打下基础。

关键词: 深色有隔内生真菌, 立枯丝核菌, 代谢组学, 差异代谢物, KEGG富集分析

Abstract:

To explore the inhibition mechanism of dark septate endophytes fungus A024 Phialocephala bamuru against Rhizoctonia solani Kühn, the non-targeted metabonomics of fungus A024 induced by R. solani were analyzed by LC-MS system. The results showed that the metabolites of A024 strain included 9 categories and 27 classes, and 52 differential metabolites were screened out. Compared with the control group, 31 of them were up-regulated and 21 of them were down-regulated after induction, among which, flavonoids, L-malic acid, guanosine monophosphate and nicotinic acid were significantly up-regulated. Arachidonic acid metabolism, biosynthesis of histidine and purine alkaloids, biosynthesis of plant secondary metabolites, biosynthesis of terpenoids and polyketide alkaloids were significantly enriched in the analysis of KEGG enrichment. Different metabolites and their enriched metabolic pathways were screened in the study, which laid a foundation for further research on the mechanism of their inhibition on R. solani.

Key words: dark septate endophytes, Rhizoctonia solani Kühn, metabonomics, differential metabolites, KEGG enrichment

中图分类号:

S763.15

宋小双, 遇文婧, 闵凯, 周琦, 邓勋, 刘艳红, 姜瑞凤. 立枯丝核菌诱导下深色有隔内生真菌A024代谢组学分析[J]. 中国农学通报, 2021, 37(8): 25-32.

Song Xiaoshuang, Yu Wenjing, Min Kai, Zhou Qi, Deng Xun, Liu Yanhong, Jiang Ruifeng. Metabonomics Analysis of Dark Septate Endophytes Phialocephala bamuru A024 Under the Induction of Rhizoctonia solani Kühn[J]. Chinese Agricultural Science Bulletin, 2021, 37(8): 25-32.

图/表 6

参考文献 28

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KEGG化合物分类		数量
脂类	脂肪酸类	19
	磷酯类	91
	糖脂类	2
	类花生酸	2
核酸	核苷	6
	核苷酸	5
	环核苷酸	2
	碱	3
抗生素	多酮类化合物与非核糖体肽	1
碳水化合物	寡糖	2
激素	神经递质	5
	其他激素	3
	肽类激素	1
	类固醇激素	7
有机酸	羧酸	13
肽类	胺类化合物	6
	氨基酸	15
	肽类	1
类固醇	十九碳	1
	二十一碳	3
	二十三碳	3
	二十四碳	5
	二十七碳	1
	二十八碳	1
	二十九碳	1
维生素和辅酶因子	辅酶因子	8
维生素和辅酶因子	维生素	6

KEGG化合物分类		数量
脂类	脂肪酸类	19
	磷酯类	91
	糖脂类	2
	类花生酸	2
核酸	核苷	6
	核苷酸	5
	环核苷酸	2
	碱	3
抗生素	多酮类化合物与非核糖体肽	1
碳水化合物	寡糖	2
激素	神经递质	5
	其他激素	3
	肽类激素	1
	类固醇激素	7
有机酸	羧酸	13
肽类	胺类化合物	6
	氨基酸	15
	肽类	1
类固醇	十九碳	1
	二十一碳	3
	二十三碳	3
	二十四碳	5
	二十七碳	1
	二十八碳	1
	二十九碳	1
维生素和辅酶因子	辅酶因子	8
维生素和辅酶因子	维生素	6

代谢物名称	保留时间/min	化学式	DSE峰面积值	DSE_SH峰面积值	变化倍数	P显著性检验
木犀草素3'-(3'-乙酰葡萄糖醛酸)	1.552883333	C₂₃H₂₀O₁₃	27.9919	140.6943	5.02	0.0003
丝氨酸	1.038916667	C₃H₇NO₃	9.156	40.5338	4.42	0.0131
GMP循环	1.038916667	C₁₀H₁₂N₅O₇P	137.8192	585.5911	4.25	0.0114
5-羟基鲍迪木醌	0.910733333	C₁₆H₁₀O₇	20.9742	82.5802	3.93	0.0009
尖福烷A	5.515016667	C₁₆H₂₂O₃	11.6882	39.1189	3.34	0.0133
磷酸鸟苷	0.910316667	C₁₀H₁₄N₅O₈P	27.6294	80.5317	2.91	0.0048
生物喋呤	1.539133333	C₉H₁₁N₅O₃	119.0244	277.5514	2.33	0.0073
L-苹果酸	1.547283333	C₄H₆O₅	404.0362	888.9781	2.20	0.0023
腺嘌呤	1.519783333	C₅H₅N₅	957.7642	1911.9471	1.99	0.0031
腺苷	1.518516667	C₁₀H₁₃N₅O₄	115.5678	228.9954	1.98	0.0041
丝氨酸内酯	1.856733333	C₁₁H₁₉NO₃	58.3043	110.4553	1.89	0.0059
烟酸	0.910733333	C₆H₅NO₂	633.4266	965.2614	1.52	0.0141
2E,6Z-金合欢醛	3.18465	C₁₅H₂₄O	473.907	397.4186	0.83	0.0333
诺龙	3.934116667	C₁₈H₂₆O₂	231.6497	187.4749	0.80	0.0443
13-酮-9Z,11E,15Z十八碳三烯酸	3.932783333	C₁₈H₂₈O₃	362.659	289.9624	0.79	0.0413
C16 鞘氨醇	5.200733333	C₁₆H₃₅NO₂	12851.11	10256.1842	0.79	0.0329
地高辛	9.105416667	C₄₁H₆₄O₁₄	122.1121	90.1886	0.73	0.0317
视黄酯	4.282716667	C₂₀H₃₀O₂	249.9794	174.0101	0.69	0.0262
5,6-环氧-8,11,14-二十碳三烯酸	4.275233333	C₂₀H₃₂O₃	618.8705	417.3965	0.67	0.0352
谷胱甘肽	0.917616667	C₁₀H₁₇N₃O₆S	282.035	183.2972	0.64	0.0260
烟酰胺	0.9451	C₆H₆N₂O	235.2482	47.185	0.20	0.0085

代谢物名称	保留时间/min	化学式	DSE峰面积值	DSE_SH峰面积值	变化倍数	P显著性检验
木犀草素3'-(3'-乙酰葡萄糖醛酸)	1.552883333	C₂₃H₂₀O₁₃	27.9919	140.6943	5.02	0.0003
丝氨酸	1.038916667	C₃H₇NO₃	9.156	40.5338	4.42	0.0131
GMP循环	1.038916667	C₁₀H₁₂N₅O₇P	137.8192	585.5911	4.25	0.0114
5-羟基鲍迪木醌	0.910733333	C₁₆H₁₀O₇	20.9742	82.5802	3.93	0.0009
尖福烷A	5.515016667	C₁₆H₂₂O₃	11.6882	39.1189	3.34	0.0133
磷酸鸟苷	0.910316667	C₁₀H₁₄N₅O₈P	27.6294	80.5317	2.91	0.0048
生物喋呤	1.539133333	C₉H₁₁N₅O₃	119.0244	277.5514	2.33	0.0073
L-苹果酸	1.547283333	C₄H₆O₅	404.0362	888.9781	2.20	0.0023
腺嘌呤	1.519783333	C₅H₅N₅	957.7642	1911.9471	1.99	0.0031
腺苷	1.518516667	C₁₀H₁₃N₅O₄	115.5678	228.9954	1.98	0.0041
丝氨酸内酯	1.856733333	C₁₁H₁₉NO₃	58.3043	110.4553	1.89	0.0059
烟酸	0.910733333	C₆H₅NO₂	633.4266	965.2614	1.52	0.0141
2E,6Z-金合欢醛	3.18465	C₁₅H₂₄O	473.907	397.4186	0.83	0.0333
诺龙	3.934116667	C₁₈H₂₆O₂	231.6497	187.4749	0.80	0.0443
13-酮-9Z,11E,15Z十八碳三烯酸	3.932783333	C₁₈H₂₈O₃	362.659	289.9624	0.79	0.0413
C16 鞘氨醇	5.200733333	C₁₆H₃₅NO₂	12851.11	10256.1842	0.79	0.0329
地高辛	9.105416667	C₄₁H₆₄O₁₄	122.1121	90.1886	0.73	0.0317
视黄酯	4.282716667	C₂₀H₃₀O₂	249.9794	174.0101	0.69	0.0262
5,6-环氧-8,11,14-二十碳三烯酸	4.275233333	C₂₀H₃₂O₃	618.8705	417.3965	0.67	0.0352
谷胱甘肽	0.917616667	C₁₀H₁₇N₃O₆S	282.035	183.2972	0.64	0.0260
烟酰胺	0.9451	C₆H₆N₂O	235.2482	47.185	0.20	0.0085

立枯丝核菌诱导下深色有隔内生真菌A024代谢组学分析

Metabonomics Analysis of Dark Septate Endophytes Phialocephala bamuru A024 Under the Induction of Rhizoctonia solani Kühn

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