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中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (8): 14-14.

所属专题： 生物技术； 畜牧兽医

• 目次 • 上一篇    下一篇

EGF对牛卵母细胞体外成熟及胚胎发育的影响

韦精卫,韦英明,孟凡丽,杨素芳,石德顺   

  • 出版日期:2006-08-05 发布日期:2006-08-05

Effects of EGF on the In vitro Maturation and Parthenogenetic Development of Bovine Oocytes

Wei Jingwei, Wei Yingming, Meng Fanli, Yang Sufang, Shi Deshun   

  • Online:2006-08-05 Published:2006-08-05

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摘要： 在卵母细胞体外成熟液（含FSH）中分别添加10，30，50ng/ml表皮生长因子（EGF），24h检查成熟率，并进行孤雌激活。结果表明：添加10ng/ml、30ng/ml EGF组牛卵母细胞成熟率较对照组（未添加EGF）无显著差异（79.8% vs 71.5% vs 70.4%，p>0.05），但EGF添加至50ng/ml时牛卵母细胞第一极体排出率显著提高，达85.4%（P<0.01）；在实验1的基础上，比较培养液中添加10ng/ml、30ng/ml 、50ng/ml EGF对牛孤雌激活胚胎体外发育的影响，结果发现在培养液中添加EGF并不能显著提高牛卵母细胞体外孤雌发育囊胚率（P>0.05），但可显著提高其囊胚孵化率（P<0.01）。

关键词: 酶抑制率, 酶抑制率, 有机磷, 氨基甲酸酯

Abstract: Effects of EGF on the in vitro maturation and parthenogenetic development of bovine oocytes were studied. The maturation rates of oocytes which cultured at maturation medium with supplementation of 10ng/ml, 30ng/ml EGF respectively had no significant different with control group (no supplementation of EGF)(79.8% vs 71.5% vs 70.4%, p>0.05), but when the EGF concentration up to 50ng/ml, the rates of Pb1 extraction (85.4%) have significant different with the control group (70.4%)(p<0.01); Supplementation of cultured media with EGF at the concentration of 10, 30, 50ng/ml didn’t cause a significant increase in the rate of blastocyst formation, however, rates of hatched blastocyst at the groups which the cultured medium supplementation with EGF were higher than control group respectively (no addition of EGF) (p<0.05).

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